Survival and conjugation of Bacillus thuringiensis in a soil microcosm.
Survival and conjugation of Bacillus thuringiensis in a soil microcosm.
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DOI：
10.1111/j.1574-6941.2000.tb00691.x
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发表时间：
2000-03
影响因子：
4.2
通讯作者：
L. Vilas-Boas;G. Vilas-Bôas;H. O. Saridakis;M. Lemos;Didier Lereclus;O. Arantes
中科院分区：
文献类型：
--
作者：
L. Vilas-Boas;G. Vilas-Bôas;H. O. Saridakis;M. Lemos;Didier Lereclus;O. Arantes
研究了苏云金芽孢杆菌和产孢子型苏云金芽孢杆菌菌株在肉汤、未改良无菌粘土单一培养和混合土壤培养中的存活和接合能力。将含有红霉素抗性决定簇和Cry1Ac基因的75kb pHT73载体转入交配液和土壤微宇宙。对菌株在土壤单一培养和混合土壤培养中的存活率进行了评估，最长可达20天。产孢菌迅速形成活的孢子，并一直保持到实验结束。产孢子菌在培养8d后不再恢复。本研究表明，产孢子型苏云金芽胞杆菌对环境的影响低于产孢型苏云金芽孢杆菌。因此，使用产孢子菌可以显著降低因苏云金芽孢杆菌生物农药在环境中传播而造成的任何潜在风险(基因转移、土壤和植物污染)。
The survival and conjugation ability of sporogenic and asporogenic Bacillus thuringiensis strains were investigated in broth, in non-amended sterile clay soil monoculture and in mixed soil culture. The 75 kb pHT73 plasmid carrying an erythromycin resistance determinant and a cry1Ac gene was transferred in mating broth and soil microcosm. Survival of strains was assessed in soil monoculture and in mixed soil culture for up to 20 days. Sporogenic strains rapidly formed viable spores which were maintained until the end of the experiment. The asporogenic strains were no longer recovered after 8 days of incubation. This study shows that the environmental impact of asporogenic B. thuringiensis strains is lower than that of sporogenic B. thuringiensis strains. Thus, the use of asporogenic strains may significantly reduce any potential risk (gene transfer, soil and plant contamination) due to the dissemination of B. thuringiensis-based biopesticides in the environment.