The Rpd3L HDAC complex is essential for the heat stress response in yeast
The Rpd3L HDAC complex is essential for the heat stress response in yeast
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DOI：
10.1111/j.1365-2958.2010.07167.x
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发表时间：
2010-05-01
影响因子：
3.6
通讯作者：
de Nadal, Eulalia
中科院分区：
文献类型：
--
作者：
Ruiz-Roig, Claudia;Vieitez, Cristina;de Nadal, Eulalia
P>为了确保细胞在温度升高期间存活和生长，真核生物会通过转录激活做出反应，从而导致蛋白质的积累，从而防止损伤并促进恢复。为了确定细胞对热应激的整体适应反应，我们进行了系统的遗传筛选，产生了高温生长所需的 277 个酵母基因。其中，Rpd3 组蛋白脱乙酰酶复合物被富集。全局基因表达分析表明，Rpd3 在热激时部分调节基因表达。 Hsf1 和 Msn2/4 转录因子是响应热应激的基因激活的主要调节因子。 RPD3缺陷细胞的Msn2/4依赖性基因的激活受损，而Hsf1控制的基因的激活不依赖于脱乙酰酶。 Rpd3 通过 Msn2/4 转录因子与热应激依赖性启动子结合，允许 RNA Pol II 进入并在应激时激活转录。最后，我们发现大的 Rpd3 复合物而不是小的 Rpd3 复合物调节细胞对热应激的适应。
P>To ensure cell survival and growth during temperature increase, eukaryotic organisms respond with transcriptional activation that results in accumulation of proteins that protect against damage and facilitate recovery. To define the global cellular adaptation response to heat stress, we performed a systematic genetic screen that yielded 277 yeast genes required for growth at high temperature. Of these, the Rpd3 histone deacetylase complex was enriched. Global gene expression analysis showed that Rpd3 partially regulated gene expression upon heat shock. The Hsf1 and Msn2/4 transcription factors are the main regulators of gene activation in response to heat stress. RPD3-deficient cells had impaired activation of Msn2/4-dependent genes, while activation of genes controlled by Hsf1 was deacetylase-independent. Rpd3 bound to heat stress-dependent promoters through the Msn2/4 transcription factors, allowing entry of RNA Pol II and activation of transcription upon stress. Finally, we found that the large, but not the small Rpd3 complex regulated cell adaptation in response to heat stress.