Fecal MicroRNAs as novel biomarkers for colon cancer screening.
Fecal MicroRNAs as novel biomarkers for colon cancer screening.
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DOI：
10.1158/1055-9965.epi-10-0027
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发表时间：
2010-07
期刊：
影响因子：
--
通讯作者：
Goel A
中科院分区：
文献类型：
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作者：
Link A;Balaguer F;Shen Y;Nagasaka T;Lozano JJ;Boland CR;Goel A
结直肠癌 (CRC) 是癌症相关死亡的第二大原因，但目前可用的非侵入性筛查项目仅实现了死亡率的小幅下降。 MicroRNA (miRNA) 在多种生物过程中发挥着重要作用，并且在肿瘤形成中通常失调。我们的目的是评估粪便 miRNA 作为结直肠肿瘤筛查生物标志物的可行性。从 8 名健康志愿者新鲜收集的粪便样本中提取总 RNA，并从正常结肠镜检查、结肠腺瘤和 CRC 受试者收集的 29 份 FOBT 粪便样本中提取总 RNA。使用 TaqMan qRT-PCR 对一部分 miRNA 进行 miRNA 表达分析。进行 Illumina miRNA 微阵列分析以评估正常结肠粘膜组织和健康受试者粪便样本之间表达模式的差异。我们使用我们开发的方案有效地从粪便样本中提取 miRNA。独立实验的数据显示 miRNA 提取和表达具有高重现性。健康志愿者粪便样本中的 miRNA 表达模式相似，并且在从同一个体连续收集的粪便样本中可重现。 29 名患者的 miRNA 表达谱表明，与无结直肠肿瘤的个体相比，腺瘤和 CRC 患者的 miR-21 和 -106a 表达更高。我们的数据表明，可以轻松且可重复地从粪便中提取 miRNA。结直肠肿瘤患者的粪便具有独特且可识别的 miRNA 表达模式。粪便 miRNA 可能是开发结直肠肿瘤非侵入性筛查测试的绝佳候选者。
Colorectal cancer (CRC) is the second leading cause of cancer-related deaths, but currently available non-invasive screening programs have achieved only a modest decrease in mortality. MicroRNAs (miRNAs) play important role in a wide array of biological processes and are commonly dysregulated in neoplasia. We aimed to evaluate the feasibility of fecal miRNAs as biomarkers for colorectal neoplasia screening. Total RNA was extracted from freshly collected stool samples from 8 healthy volunteers and 29 FOBT collected feces from subjects with normal colonoscopies, colon adenomas and CRCs. miRNA expression analysis were performed with TaqMan qRT-PCR for a subset of miRNAs. Illumina miRNA microarray profiling was performed to evaluate the differences in expression patterns between normal colonic mucosa tissues and stool samples from healthy subjects. We efficiently extracted miRNAs from stool specimens using our developed protocol. Data from independent experiments showed high reproducibility for miRNA extraction and expression. miRNA expression patterns were similar in stool specimens among healthy volunteers and reproducible in stool samples that were collected serially in time from the same individuals. miRNA expression profiles from 29 patients demonstrated higher expression of miR-21 and -106a in patients with adenomas and CRCs, compared with individuals free of colorectal neoplasia. Our data indicate that miRNAs can be extracted from stool easily and reproducibly. The stools of patients with colorectal neoplasms have unique and identifiable patterns of miRNA expression. Fecal miRNAs may be an excellent candidate for the development of a non-invasive screening test for colorectal neoplasms.