ODORANT-BINDING-PROTEIN SUBFAMILIES ASSOCIATE WITH DISTINCT CLASSES OF OLFACTORY RECEPTOR NEURONS IN INSECTS
ODORANT-BINDING-PROTEIN SUBFAMILIES ASSOCIATE WITH DISTINCT CLASSES OF OLFACTORY RECEPTOR NEURONS IN INSECTS
复制标题
DOI：
10.1002/neu.480220108
复制
发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
LERNER, MR
中科院分区：
文献类型：
--
作者：
VOGT, RG;PRESTWICH, GD;LERNER, MR
陆生动物的嗅觉受体存在于水环境中，但检测到的气味主要是疏水性的。 疏水性气味剂的水溶性被认为通过存在于气味剂受体周围的细胞外液中的气味剂结合蛋白（OBP）而大大增强。 我们从 6 种昆虫（蛾）物种中分离出 14 个候选 OBP，并对其进行了部分测序。 所有 14 个代表基于保守序列域的单一同源家族。 根据组织特异性表达的差异和氨基酸序列的相似性，这 14 种蛋白质可分为三个亚家族。 所有 14 种蛋白质均在触角嗅觉组织中特异性表达。 亚家族 I 代表先前描述的信息素结合蛋白 (PBP)，它们是雄性特异性的，与信息素敏感神经元相关，并且在物种之间比较时其序列高度可变。 亚科 II 和 III 在雄性和雌性触角中表达，似乎与一般气味敏感神经元相关，并且在物种间比较时高度保守。 亚家族 II 和 III 蛋白的特性表明它们是一般气味结合蛋白 (GOBP)。 各个昆虫 OBP 亚科的特性表明它们具有不同的气味结合特异性。 不同昆虫 OBP 亚科与具有不同气味特异性的不同类别嗅觉神经元的关联表明，OBP 可以充当实际受体蛋白外围的选择性信号过滤器。
The olfactory receptors of terrestrial animals exist in an aqueous environment, yet detect odorants that are primarily hydrophobic. The aqueous solubility of hydrophobic odorants is thought to be greatly enhanced via odorant binding proteins (OBP) which exist in the extracellular fluid surrounding the odorant receptors. We have isolated and partially sequenced 14 candidate OBPs from six insect (moth) species. All 14 represent a single homologous family based on conserved sequence domains. The 14 proteins can be divided into three subfamilies based on differences in tissue specific expression and similarities in amino acid sequences. All 14 proteins are specifically expressed in antennal olfactory tissue. Subfamily I represents previously described pheromone binding proteins (PBP), which are male-specific, associate with pheromone-sensitive neurons, and are highly variable in their sequences when compared among species. Subfamilies II and III are expressed in both male and female antennae, appear to associate with general-odorant-sensitive neurons, and are highly conserved when compared among species. The properties of the subfamily II and III proteins suggest these are general-odorant binding proteins (GOBP). The properties of the respective insect OBP subfamilies suggest that they have different odorant binding specificities. The association of different insect OBP subfamilies with distinct classes of olfactory neurons having different odorant specificities suggests that OBPs can act as selective signal filters, peripheral to the actual receptor proteins.