Evaluation of the anti-allergic activity of Citrus unshiu using rat basophilic leukemia RBL-2H3 cells as well as basophils of patients with seasonal allergic rhinitis to pollen.
Evaluation of the anti-allergic activity of Citrus unshiu using rat basophilic leukemia RBL-2H3 cells as well as basophils of patients with seasonal allergic rhinitis to pollen.
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DOI：
10.3892/ijmm.17.3.511
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发表时间：
2006-03
影响因子：
5.4
通讯作者：
Shoko Kobayashi;S. Tanabe
中科院分区：
文献类型：
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作者：
Shoko Kobayashi;S. Tanabe
研究了50%甲醇提取物（MEC）的抗过敏活性。香柏木粉50%甲醇提取物对季节性变应性鼻炎患者嗜碱性细胞对香柏木花粉的组胺释放有较强的抑制作用。为了详细研究这种抗过敏机制，我们接下来使用大鼠嗜碱性白血病RBL-2H3细胞。MEC显著抑制ige诱导的RBL-2H3细胞组胺和β -己糖氨酸酶释放。由于MEC含有多种黄酮类化合物，我们选择了nobiletin、橙皮苷和橙皮苷（橙皮苷苷）作为代表化合物，并进一步评估了这些化合物的抑制活性。在测试的黄酮类化合物中，橙皮苷是最有效的，而橙皮苷的抑制活性要小得多，如果有的话。然后研究了黄酮类化合物抑制脱粒过程的机制。因此，橙皮素和去黄皮素抑制了磷脂酰肌醇3-激酶（PI3-K）的直接下游效应因子Akt-1的磷酸化。因此，我们认为这些类黄酮通过PI3-K途径抑制ige介导的嗜碱性细胞刺激，适当摄入柑橘有利于控制香柏花粉引起的季节性变应性鼻炎。
The anti-allergic activity of the 50% methanol extract of Citrus unshiu powder (MEC) was examined. Fifty percent methanol extract of MEC powder showed potent inhibitory activity against histamine release from basophils of patients suffering from seasonal allergic rhinitis to ceder pollen. To examine this anti-allergic mechanism in detail, we next used rat basophlilic leukemia RBL-2H3 cells. MEC significantly inhibited IgE-induced histamine and beta-hexosaminidase release from RBL-2H3 cells. Since MEC contains a variety of flavonoids, we selected nobiletin, hesperetin, and hesperidin (hesperetin glycoside) as representative compounds, and further evaluated these inhibitory activities. Among the flavonoids tested, hesperetin was the most potent, while hesperidin had far less, if any, inhibitory activity. The mechanism by which flavonoids inhibited the degranulation process was then examined. As a result, hesperetin and nobiletin suppressed the phosphorylation of Akt-1, direct downstream effector of phosphatidylinositol 3-kinase (PI3-K). Thus, it was assumed that these flavonoids suppressed IgE-mediated stimulation of basophils through PI3-K pathway and that proper intake of Citrus unshiu would be favorable for managing seasonal allergic rhinitis to ceder pollen.