Cells containing IgE in the intestinal mucosa of mice infected with the nematode parasite Trichinella spiralis are predominantly of a mast cell lineage.
Cells containing IgE in the intestinal mucosa of mice infected with the nematode parasite Trichinella spiralis are predominantly of a mast cell lineage.
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DOI：
10.4049/jimmunol.137.8.2555
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发表时间：
1986-10
影响因子：
4.4
通讯作者：
H. Alizadeh;J. Urban;I. Katona;F. Finkelman
中科院分区：
文献类型：
--
作者：
H. Alizadeh;J. Urban;I. Katona;F. Finkelman
为了确定线虫感染小鼠肠粘膜中的IgE+细胞是肥大细胞还是淋巴细胞谱系，在接种旋毛虫肌肉期幼虫后检查肥大细胞缺陷的w/wv小鼠肠粘膜中的IgE+细胞。免疫荧光染色技术用于检测与肠粘膜细胞相关的IgE。在未感染对照或接种T.螺旋形的取T.将螺旋体感染的BALB/c、SJL和+/+小鼠固定在乙醇中，并用亲和纯化的F（ab ′）2兔抗小鼠IgE染色，然后用FITC山羊抗兔IgG染色。肠粘膜中大量细胞显示明亮的荧光。当这些小鼠的其他肠切片在Carnoy固定液中处理，并在低pH下用阿辛蓝（肥大细胞的异染性染色）染色或用阿辛蓝染色，然后进行IgE免疫荧光染色时，在肠粘膜中观察到大量肥大细胞，70 - 90%的IgE染色阳性。肠粘膜中有相当数量的IgE+细胞，但未被阿辛蓝染色。在对照、未感染+/+、BALB/c和SJL小鼠的肠粘膜中存在少量阿辛蓝阳性细胞和无IgE+染色细胞。为了确定这些IgE+阿新蓝阴性细胞是淋巴细胞还是肥大细胞谱系，在用T.螺旋形的与BALB/c、SJL或+/+小鼠相比，T.螺旋体感染的w/wv小鼠用阿辛蓝染色或对IgE呈阳性。然而，当将w/wv小鼠MLN中的IgE反应与BALB/c、SJL和+/+小鼠的IgE反应进行比较时，在所有四种T.感染螺旋体的小鼠。此外，对MLN细胞悬浮液中表面IgE染色的流式显微荧光分析显示，在所有四种T细胞株中，携带IgE的细胞（主要是B淋巴细胞）比例相当。感染螺旋体的小鼠。（400字处删节）
To determine whether IgE+ cells in the intestinal mucosa of nematode-infected mice were of a mast cell or a lymphocyte lineage, the intestinal mucosae of mast cell-deficient w/wv mice were examined for IgE+ cells after inoculation with Trichinella spiralis muscle-stage larvae. Immunofluorescence staining techniques were used to detect IgE associated with cells in the intestinal mucosa. Comparisons were made among four strains of mice, w/wv (mast cell-deficient), +/+ (normal congenic littermates of w/wv), BALB/c, and SJL, that were either uninfected controls or inoculated with T. spiralis. Tissue sections from the small intestine of T. spiralis-infected BALB/c, SJL, and +/+ mice were fixed in ethanol and were stained with an affinity-purified F(ab')2 rabbit anti-mouse IgE followed by FITC goat anti-rabbit IgG. Large numbers of cells in the intestinal mucosa exhibited bright fluorescence. When other sections of intestines from these mice were processed in Carnoy's fixative and were stained with alcian blue at low pH (a metachromatic stain for mast cells) or alcian blue followed by immunofluorescence staining for IgE, large numbers of mast cells were observed in the intestinal mucosa, and 70 to 90% stained positively for IgE. There was a considerable number of cells in the intestinal mucosa which were IgE+ but which did not stain with alcian blue. Few alcian blue-positive cells and no IgE+ staining cells were present in the intestinal mucosa of control, uninfected +/+, BALB/c, and SJL mice. To determine whether these IgE+ alcian blue-negative cells were of a lymphocyte or a mast cell lineage, the mast cell-deficient w/wv mouse strain was examined after infection with T. spiralis. In contrast to BALB/c, SJL, or +/+ mice, few cells in the intestinal mucosa of T. spiralis-infected w/wv mice stained with alcian blue or were positive for IgE. However, when the IgE response in the MLN of the w/wv mice was compared to the IgE response of BALB/c, SJL, and +/+ mice, numerous IgE+ cells, but no alcian blue-positive cells, were observed in the parenchyma of the MLN from all four strains of T. spiralis-infected mice. In addition, flow microfluorometric analysis of MLN cells stained for surface IgE in suspension showed a comparable proportion of IgE-bearing cells, which were mostly B lymphocytes, among all four strains of T. spiralis-infected mice.(ABSTRACT TRUNCATED AT 400 WORDS)