Bioconjugation and Applications of Amino Functional Fluorescence Polymers
Bioconjugation and Applications of Amino Functional Fluorescence Polymers
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DOI：
10.1002/mabi.201600232
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发表时间：
2017-03-01
影响因子：
4.6
通讯作者：
Yagci, Yusuf
中科院分区：
文献类型：
--
作者：
Geyik, Caner;Guler, Emine;Yagci, Yusuf
用于诊断和治疗的生物功能聚合物的合成和新应用是涉及各个研究领域的有前途的领域。在此，合成了三种荧光聚合物：聚（对亚苯基-噻吩）、聚（对亚苯基）和带有氨基的聚噻吩（分别为 PPT-NH2、PPP-NH2 和 PT-NH2），并研究其用于癌细胞靶向成像、药物递送和放射治疗。聚合物与抗 HER2 抗体缀合，用于无毒浓度的靶向成像研究。使用具有不同 HER2 表达水平的三种细胞系（A549、Vero 和 HeLa）。在表达 HER2 的细胞系 (A549) 模型中进行放射治疗实验，结果表明所有三种聚合物都提高了放射治疗的功效。当与抗 HER2 缀合时，这种效果甚至会进一步增强。在这项工作的第二部分中，一种选定的聚合物（PT-NH2）与药物模型缀合；甲氨蝶呤通过 pH 响应腙键和 PT-NH2 的药物载体特性在神经母细胞瘤 (SH-SY5Y) 细胞模型中得到证实。我们的结果表明，PPT-NH2、PPP-NH2 和 PT-NH2 作为生物材料在癌症研究的各种生物应用中具有巨大的潜力。
Synthesis and novel applications of biofunctional polymers for diagnosis and therapy are promising area involving various research domains. Herein, three fluorescent polymers, poly(p-phenylene-co-thiophene), poly(p-phenylene), and polythiophene with amino groups (PPT-NH2, PPP-NH2, and PT-NH2, respectively) are synthesized and investigated for cancer cell targeted imaging, drug delivery, and radiotherapy. Polymers are conjugated to anti-HER2 antibody for targeted imaging studies in nontoxic concentrations. Three cell lines (A549, Vero, and HeLa) with different expression levels of HER2 are used. In a model of HER2 expressing cell line (A549), radiotherapy experiments are carried out and results show that all three polymers increase the efficacy of radiotherapy. This effect is even more increased when conjugated to anti-HER2. In the second part of this work, one of the selected polymers (PT-NH2) is conjugated with a drug model; methotrexate via pH responsive hydrazone linkage and a drug carrier property of PT-NH2 is demonstrated on neuroblastoma (SH-SY5Y) cell model. Our results indicate that, PPT-NH2, PPP-NH2, and PT-NH2 have a great potential as biomaterials for various bioapplications in cancer research.