Methylopila jiangsuensis sp. nov., an aerobic, facultatively methylotrophic bacterium.
Methylopila jiangsuensis sp. nov., an aerobic, facultatively methylotrophic bacterium.
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DOI：
10.1099/ijs.0.020925-0
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发表时间：
2011-07
影响因子：
2.8
通讯作者：
Lian-tai Li;Jin-Wei Zheng;Bao-jian Hang;N. Doronina;Y. Trotsenko;Jian He;Shun-Peng Li
中科院分区：
文献类型：
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作者：
Lian-tai Li;Jin-Wei Zheng;Bao-jian Hang;N. Doronina;Y. Trotsenko;Jian He;Shun-Peng Li
确定了从活性污泥中分离出的需氧、兼性甲基营养菌株 JZL-4(T) 的分类状态。细胞为革兰氏阴性、无孢子、无色、能运动、短杆状。该菌株利用甲醇、甲胺、甲酸盐和多种多碳化合物作为碳源和能源，但不利用甲烷、二氯甲烷或CO(2)/H(2)。 C(1) 化合物通过异柠檬酸裂解酶阴性丝氨酸途径同化。最佳生长条件为 30 °C、pH 6.5-7.5 和 0.5 % (w/v) NaCl。主要的细胞脂肪酸是C(18:1)ω7c和C(18:0)。主要的磷脂是磷脂酰乙醇胺、磷脂酰胆碱、磷脂酰甘油和磷脂酰单甲基乙醇胺(PME)； PME 是菌株 JZL-4(T) 的主要磷脂，但在荚膜甲基菌 IM1(T)、赫尔维菌甲基菌 DM9(T) 和食甲基白杆菌 DM10(T) 中不存在或仅少量存在。主要的泛醌是 Q-10。菌株JZL-4(T)的DNA G+C含量为70.4mol％(T(m))。基于16S rRNA基因序列的系统发育分析表明，该菌株与M. capsulata IM1(T) (97.2 %)、A.methylovorans DM10(T) (94.9 %)和M. helvetica DM9(T) (94.1 %)具有较高的序列相似性，与其他有效公布名称的菌株相似性低于94 %。菌株 JZL-4(T) 与 M. capsulata IM1(T) 的 DNA-DNA 相关性水平较低 (34%)。根据表型、遗传和系统发育数据，菌株JZL-4(T)被认为是甲基毛菌属的一个新种，命名为江苏甲基毛菌。十一月典型菌株是菌株 JZL-4(T) ( = ACCC 05406(T) = DSM 22718(T) = VKM B-2555(T))。
The taxonomic status was determined of an aerobic, facultatively methylotrophic strain, JZL-4(T), isolated from activated sludge. The cells were gram-negative, asporogenous, colourless, motile, short rods. The strain utilized methanol, methylamine, formate and a variety of polycarbon compounds, but not methane, dichloromethane or CO(2)/H(2), as carbon and energy sources. C(1) compounds were assimilated via the isocitrate lyase-negative serine pathway. Optimal growth occurred at 30 °C, pH 6.5-7.5 and 0.5 % (w/v) NaCl. The major cellular fatty acids were C(18 : 1)ω7c and C(18 : 0). The major phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylmonomethylethanolamine (PME); PME, the main phospholipid of strain JZL-4(T), was absent or present in only minor amounts in Methylopila capsulata IM1(T), Methylopila helvetica DM9(T) and Albibacter methylovorans DM10(T). The major ubiquinone was Q-10. The DNA G+C content of strain JZL-4(T) was 70.4 mol% (T(m)). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain showed high sequence similarities to M. capsulata IM1(T) (97.2 %), A. methylovorans DM10(T) (94.9 %) and M. helvetica DM9(T) (94.1 %), and showed less than 94 % similarity to strains of other species with validly published names. Strain JZL-4(T) had a low level of DNA-DNA relatedness (34 %) with M. capsulata IM1(T). On the basis of phenotypic, genetic and phylogenetic data, strain JZL-4(T) is proposed to represent a novel species of the genus Methylopila, with the name Methylopila jiangsuensis sp. nov. The type strain is strain JZL-4(T) ( = ACCC 05406(T) = DSM 22718(T) = VKM B-2555(T)).