Selected fructooligosaccharide (1-kestose, nystose, and 1(F)-beta-fructofuranosylnystose) composition of foods and feeds
Selected fructooligosaccharide (1-kestose, nystose, and 1(F)-beta-fructofuranosylnystose) composition of foods and feeds
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DOI：
10.1021/jf970087g
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发表时间：
1997-08-01
影响因子：
6.1
通讯作者：
Hunter, DE
中科院分区：
文献类型：
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作者：
Campbell, JM;Bauer, LL;Hunter, DE
低聚果糖（FOS）是天然存在的糖，具有潜在的有益营养作用。它们广泛分布于植物界。建立了一种离子色谱法，用于快速准确地测定人类和动物食用的选定食品和饲料成分中的FOS。本研究的目的是测定各种食品和饲料中1-酮糖（1-酮三糖；GF(2)）、糖糖（1,1-酮四糖；GF(3)）和1(F)- β -果糖呋喃糖糖（1,1,1-酮二糖；GF(4)）的含量。经水萃取和适当过滤后，使用碱性醋酸钠梯度，通过离子交换柱和配有脉冲电化学检测器的Dionex色谱单元的保护装置进行色谱分析。所有样品均制备有和没有标准峰，以验证回收率和峰识别。菊科样品的总FOS最高，其次是苋菜科的葱属植物。该方法对FOS的GF(n)单位提供了良好的分离、回收和定量。FOS的准确定量将允许开发更精确的营养配方，将这种功能性食品成分纳入人类和动物的饮食中。
Fructooligosaccharides (FOS) are naturally occurring sugars with potentially beneficial nutritional effects. They are widely distributed throughout the plant kingdom. An ion chromatographic method was developed to rapidly and accurately measure FOS in selected food and feed ingredients ingested by humans and animals. The objective of this study was to determine the 1-kestose (1-kestotriose; GF(2)), nystose (1,1-kestotetraose; GF(3)), and 1(F)-beta-fructofuranosylnystose (1,1,1-kestopentaose; GF(4)) content of a wide variety of foods and feedstuffs. After extraction with water and appropriate filtration, samples were chromatographed, using an alkaline sodium acetate gradient, through an ion exchange column and guard fitted to a Dionex chromatography unit equipped with a pulsed electrochemical detector. All samples were prepared both with and without spikes of standards to verify recovery and peak identification. Samples of the Compositae family were highest in total FOS followed by Allium species of the Amaryllidadeae family. The method provided excellent separation, recovery, and quantification of the GF(n) units of FOS. Accurate quantitation of FOS will allow more precise nutritional formulations to be developed with respect to inclusion of this functional food component in human and animal diets.