Transcriptome profiling of sulfate deprivation responses in two agarophytes Gracilaria changii and Gracilaria salicornia (Rhodophyta).
Transcriptome profiling of sulfate deprivation responses in two agarophytes Gracilaria changii and Gracilaria salicornia (Rhodophyta).
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DOI：
10.1038/srep46563
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发表时间：
2017-04-24
影响因子：
4.6
通讯作者：
Ho CL
中科院分区：
文献类型：
--
作者：
Lee WK;Namasivayam P;Ong Abdullah J;Ho CL
海藻生存在海洋沃茨与高硫酸盐浓度相比，那些生活在淡水栖息地。江蓠细胞壁聚合物。供应世界50%以上的琼脂是严重硫酸化的。由于硫酸化降低了琼脂的质量，这是有趣的，调查硫酸盐剥夺对海藻和琼脂的硫酸盐含量的影响，以及这些海藻的代谢途径。本研究以两种琼胶植物G. changii和G.盐角草在硫酸盐剥夺下处理5天。去除硫酸盐后，海藻/琼脂中硫酸盐含量普遍低于对照组，但只有G. changii和G.海蓬子RNA测序（RNA-Seq）的硫酸盐剥夺和未处理的海藻样品显示1,292和3,439差异表达的基因（DEG; ≥1.5倍）在硫酸盐剥夺G。changii和G.海蓬子，分别与各自的控制。在注释的DEG中，涉及推定的琼脂生物合成、硫代谢、含硫氨基酸代谢、碳代谢和氧化应激的基因。这些发现揭示了琼胶植物中硫酸盐剥夺反应，并有助于确定参与琼胶生物合成的候选基因。
Seaweeds survive in marine waters with high sulfate concentration compared to those living at freshwater habitats. The cell wall polymer of Gracilaria spp. which supplies more than 50% of the world agar is heavily sulfated. Since sulfation reduces the agar quality, it is interesting to investigate the effects of sulfate deprivation on the sulfate contents of seaweed and agar, as well as the metabolic pathways of these seaweeds. In this study, two agarophytes G. changii and G. salicornia were treated under sulfate deprivation for 5 days. The sulfate contents in the seaweed/agar were generally lower in sulfate-deprivated samples compared to those in the controls, but the differences were only statistically significant for seaweed sample of G. changii and agar sample of G. salicornia. RNA sequencing (RNA-Seq) of sulfate-deprivated and untreated seaweed samples revealed 1,292 and 3,439 differentially expressed genes (DEGs; ≥1.5-fold) in sulfate-deprivated G. changii and G. salicornia, respectively, compared to their respective controls. Among the annotated DEGs were genes involved in putative agar biosynthesis, sulfur metabolism, metabolism of sulfur-containing amino acids, carbon metabolism and oxidative stress. These findings shed light on the sulfate deprivation responses in agarophytes and help to identify candidate genes involved in agar biosynthesis.