Dielectrophoretic manipulation of particles
Dielectrophoretic manipulation of particles
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DOI：
10.1109/ias.1995.530460
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发表时间：
1995-10
期刊：
影响因子：
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通讯作者：
Xiao-Bo Wang;Ying Huang;P. Gascoyne;Frederick F. Becker
中科院分区：
文献类型：
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作者：
Xiao-Bo Wang;Ying Huang;P. Gascoyne;Frederick F. Becker
作者最近证明，通常情况下，粒子所经历的介电泳力有两个分量。第一个依赖于场强的不均匀性和外加电场在粒子中诱导的偶极矩的同相部分。这一成分可以用常规的介电泳力(CDEP)来识别。第二个分量与场相的不均匀分布和感应偶极矩的不同相部分有关。不均匀的相位分布对应于穿过空间的场。这种第二种力分量产生了行波介质电泳(TwDEP)。在本文中，作者描述了几种设计用于产生电场的电极结构，这些电场能够诱导CDEP末端的两个DEP作用力，以达到操纵颗粒的目的。以介电特性良好的小鼠红白血病细胞为测试粒子，研究了这些电极的电动力学行为随频率和电极激发方式的变化。根据电极的激发特性和电池的介电性质(包括捕获电池的直线运动、悬浮和循环)，确定了几种特征的电池电动行为。他们描述了这些发现，并根据电极产生的场分布和广义介电理论使其合理化。然后讨论了介电操作的生物技术应用。
The authors have demonstrated recently that, in general, the dielectrophoretic (DEP) force experienced by a particle has two components. The first depends upon inhomogeneities in the field strength and upon the in-phase part of the dipole moment induced in the particle by the applied electric field. This component can be identified with the conventional dielectrophoretic (cDEP) force. The second component relates to the nonuniform distribution of the phase of the field and to the out-of-phase part of the induced dipole moment. A nonuniform phase distribution corresponds to the field traveling through space. This second force component gives rise to traveling wave dielectrophoresis (twDEP). In this paper, the authors describe several electrode configurations designed to produce electric fields capable of inducing cDEP end twDEP forcer for the purpose of manipulating particles. Using DS19 Friend murine erythroleukemia cells as test particles with well characterized dielectric properties, they investigated the electrokinetic behaviors for these electrodes as a function of frequency and electrode excitation mode. Several characteristic cell electrokinetic behaviors were identified depending on the excitation characteristics of the electrodes and the cell dielectric properties including trapping linear motion, levitation and circulation of the cells. They describe these findings and rationalize them in terms of field distributions produced by the electrodes and generalized dielectrophoresis theory. The biotechnological applications of dielectrophoretic manipulation are then discussed.