Dimorphism in genes encoding sexual-stage proteins of Plasmodium ovale curtisi and Plasmodium ovale wallikeri.
Dimorphism in genes encoding sexual-stage proteins of Plasmodium ovale curtisi and Plasmodium ovale wallikeri.
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编码质量卵形柯蒂西和卵子质质质量的基因中的二态性。
DOI：
10.1016/j.ijpara.2015.02.004
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发表时间：
2015-06
影响因子：
4
通讯作者：
Sutherland, Colin J.
中科院分区：
文献类型：
--
作者：
Oguike, Mary C.;Sutherland, Colin J.
我们提出了卵圆疟原虫性阶段基因的新序列数据，这些基因证明了两种卵圆疟原虫之间存在实质性的二态性。本研究结果为卵形疟原虫之间的非重组提供了进一步的证据，为进一步开展卵形疟原虫的生物学研究提供了平台。柯氏卵圆疟原虫和瓦利克氏卵圆疟原虫是不同种类的疟疾寄生虫，除美洲外，它们在整个热带地区都是同域的。尽管这两个物种在地理范围上完全重叠，但它们并没有重新组合。虽然在形态上非常相似，但两个分类群必须具有不同的特征，以防止它们之间的重组。我们假设，有性生殖所需的蛋白质在两种蚊子之间已经充分分化，以防止在摄入两种蚊子配子体的任何蚊子血液中重组。为了探究卵形疟原虫curtisi和卵形疟原虫walllikeri种间交配可能存在的障碍，我们在其他疟原虫中鉴定了编码性期蛋白的同源基因，并对这两种疟原虫进行了比较。在现有的卵形疟原虫部分基因组数据库（Wellcome Trust Sanger Institute， UK）中进行了6-半胱氨酸、limus凝血因子C结构域蛋白和其他相关性期蛋白的数据库检索。获得的序列片段作为PCR沿着每个感兴趣的基因行走的基础，在两个卵形圆孔线虫和卵形圆孔线虫的参考分离物。对各物种各基因同源物的序列比对显示，所有分离株均具有完全的二态性。总之，观察到两种卵形瓢虫性期蛋白之间存在实质性差异，进一步证明它们在自然界中不会重组。因此，参与性发育和受精的蛋白质的不相容仍然是一个合理的解释，可以观察到在卵形矮种猿和卵形矮种猿之间缺乏自然重组。
We present novel sequence data for putative sexual stage genes in Plasmodium ovale spp. Substantial dimorphism between the two Plasmodium ovale spp. is demonstrated by these genes. Our data provide further evidence for non-recombination between ovale spp. This information provides a platform for further research into the biology of ovale malaria. Plasmodium ovale curtisi and Plasmodium ovale wallikeri are distinct species of malaria parasite which are sympatric throughout the tropics, except for the Americas. Despite this complete overlap in geographic range, these two species do not recombine. Although morphologically very similar, the two taxa must possess distinct characters which prevent recombination between them. We hypothesised that proteins required for sexual reproduction have sufficiently diverged between the two species to prevent recombination in any mosquito blood meal in which gametocytes of both species are ingested. In order to investigate possible barriers to inter-species mating between P. ovale curtisi and P. ovale wallikeri, homologues of genes encoding sexual stage proteins in other plasmodia were identified and compared between the two species. Database searches with motifs for 6-cysteine, Limulus Coagulation factor C domain-containing proteins and other relevant sexual stage proteins in the genus Plasmodium were performed in the available P. ovale curtisi partial genome database (Wellcome Trust Sanger Institute, UK). Sequence fragments obtained were used as the basis for PCR walking along each gene of interest in reference isolates of both P. ovale curtisi and P. ovale wallikeri. Sequence alignment of the homologues of each gene in each species showed complete dimorphism across all isolates. In conclusion, substantial divergence between sexual stage proteins in the two P. ovale spp. was observed, providing further evidence that these do not recombine in nature. Incompatibility of proteins involved in sexual development and fertilisation thus remains a plausible explanation for the observed lack of natural recombination between P. ovale curtisi and P. ovale wallikeri.
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