ISOLATION OF HUMAN LYMPHOCYTES-T - COMPARISON BETWEEN NYLON WOOL FILTRATION AND ROSETTING WITH NEURAMINIDASE (VCN) AND 2-AMINOETHYLISOTHIOURONIUM BROMIDE (AET)-TREATED SHEEP RED-BLOOD-CELLS (SRBC)
ISOLATION OF HUMAN LYMPHOCYTES-T - COMPARISON BETWEEN NYLON WOOL FILTRATION AND ROSETTING WITH NEURAMINIDASE (VCN) AND 2-AMINOETHYLISOTHIOURONIUM BROMIDE (AET)-TREATED SHEEP RED-BLOOD-CELLS (SRBC)
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DOI：
10.1016/0022-1759(80)90164-7
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发表时间：
1980-01-01
影响因子：
2.2
通讯作者：
FERRONE, S
中科院分区：
文献类型：
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作者：
INDIVERI, F;HUDDLESTONE, J;FERRONE, S
通过用 SRBC（羊红细胞）玫瑰花结法分离人 T（胸腺来源）淋巴细胞，在聚蔗糖-泛影葡胺梯度上离心并裂解 SRBC（据报道影响淋巴细胞的功能活性），与通过尼龙毛柱过滤分离 T 淋巴细胞进行比较。两种方法的 T 淋巴细胞产量和纯度相似。根据T.gamma的百分比判断，通过尼龙毛柱过滤分离的T淋巴细胞中没有发现T细胞亚群的选择性损失。和T.mu。以及功能测定中的抑制和辅助活性。因此，通过尼龙毛柱过滤是一种有用的纯化T淋巴细胞的方法，并且具有相对简单和快速的优点，并且避免了可能影响T淋巴细胞功能活性的操作。
Isolation of human T [thymus-derived] lymphocytes by rosetting with SRBC [sheep red blood cells] centrifugation on Ficoll-Hypaque gradients and lysis of SRBC, which reportedly affects the functional activities of lymphocytes, was compared to isolation of T lymphocytes by filtration through a nylon wool column. The 2 methods were similar in yields and purity of T lymphocytes. No selective loss of T cell subpopulations was found in T lymphocytes isolated by filtration through a nylon wool column as judged by the percentage of T.gamma. and T.mu. and the suppressor and helper activity in functional assays. Therefore, filtration through a nylon wool column is a useful method to purify T lymphocytes and has the advantage of being comparatively simple and quick to perform and of avoiding manipulations which may affect the functional activity of T lymphocytes.