Indoor Dust as a Matrix for Surveillance of COVID-19.
Indoor Dust as a Matrix for Surveillance of COVID-19.
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室内灰尘作为矩阵，用于监视COVID-19。
DOI：
10.1128/msystems.01350-20
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发表时间：
2021-04-13
期刊：
影响因子：
6.4
通讯作者：
Dannemiller KC
中科院分区：
文献类型：
--
作者：
Renninger N;Nastasi N;Bope A;Cochran SJ;Haines SR;Balasubrahmaniam N;Stuart K;Bivins A;Bibby K;Hull NM;Dannemiller KC
持续的疾病监测是减轻病毒爆发的关键工具，特别是在大流行期间。即使在高危人群中广泛接种疫苗之后，环境监测也有很大的希望。这项工作的目标是证明分子严重急性呼吸综合征冠状病毒2（SARS-CoV-2）监测散装地板灰尘和相关样本作为一种非侵入性环境监测方法的概念证明2019冠状病毒病（COVID-19）和潜在的其他病毒性疾病。从COVID-19阳性个体的房间中采集表面拭子、被动采样器和大量地板灰尘样本，并采用定量逆转录PCR（RT-qPCR）和两种数字PCR（dPCR）方法检测SARS-CoV-2。散装粉尘样品的几何平均浓度为163拷贝/毫克粉尘，范围从未检测到23，049拷贝/毫克粉尘使用液滴数字PCR（ddPCR）检测。平均89%的散装灰尘样本的检测方法呈阳性的病毒相比，55%的表面拭子和被动采样器（19%地毯，29%聚苯乙烯）较少。在散装粉尘中，通过qPCR、基于芯片的dPCR和液滴dPCR分别在76%、93%和97%的样品中检测到SARS-CoV-2。尽管在房间清洁前使用了消毒剂，但散装真空袋中可检测到的病毒RNA在4周内未发生可测量的衰减。未来的监测工作应进一步评估尘埃中RNA的持续性和异质性。这项研究没有测量灰尘中的病毒感染性或与灰尘相关的潜在传播。总的来说，这项工作表明，散装地板灰尘是一个潜在的有用的矩阵长期监测病毒性疾病的高风险人群和建筑物。重要性评估社区内病原体存在的环境监测被证明是保护公共卫生的关键工具，在COVID-19大流行期间尤其重要。重要的是，环境监测工具还可以检测无症状疾病携带者，并对大量人群进行常规监测，如SARS-CoV-2废水监测所示。然而，需要更多的监测技术来筛查在高风险环境中的爆发，如集体护理设施。在这里，我们证明，SARS-CoV-2可以检测到散装地板灰尘收集从房间感染的个人。这一分析表明，灰尘可能是一个有用的和有效的矩阵，用于病毒性疾病的常规监测。
Ongoing disease surveillance is a critical tool to mitigate viral outbreaks, especially during a pandemic. Environmental monitoring has significant promise even following widespread vaccination among high-risk populations. The goal of this work is to demonstrate molecular severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) monitoring in bulk floor dust and related samples as a proof of concept of a noninvasive environmental surveillance methodology for coronavirus disease 2019 (COVID-19) and potentially other viral diseases. Surface swab, passive sampler, and bulk floor dust samples were collected from the rooms of individuals positive for COVID-19, and SARS-CoV-2 was measured with quantitative reverse transcription-PCR (RT-qPCR) and two digital PCR (dPCR) methods. Bulk dust samples had a geometric mean concentration of 163 copies/mg of dust and ranged from nondetects to 23,049 copies/mg of dust detected using droplet digital PCR (ddPCR). An average of 89% of bulk dust samples were positive for the virus by the detection methods compared to 55% of surface swabs and fewer on the passive sampler (19% carpet, 29% polystyrene). In bulk dust, SARS-CoV-2 was detected in 76%, 93%, and 97% of samples measured by qPCR, chip-based dPCR, and droplet dPCR, respectively. Detectable viral RNA in the bulk vacuum bags did not measurably decay over 4 weeks, despite the application of a disinfectant before room cleaning. Future monitoring efforts should further evaluate RNA persistence and heterogeneity in dust. This study did not measure virus infectivity in dust or potential transmission associated with dust. Overall, this work demonstrates that bulk floor dust is a potentially useful matrix for long-term monitoring of viral disease in high-risk populations and buildings. IMPORTANCE Environmental surveillance to assess pathogen presence within a community is proving to be a critical tool to protect public health, and it is especially relevant during the ongoing COVID-19 pandemic. Importantly, environmental surveillance tools also allow for the detection of asymptomatic disease carriers and for routine monitoring of a large number of people as has been shown for SARS-CoV-2 wastewater monitoring. However, additional monitoring techniques are needed to screen for outbreaks in high-risk settings such as congregate care facilities. Here, we demonstrate that SARS-CoV-2 can be detected in bulk floor dust collected from rooms housing infected individuals. This analysis suggests that dust may be a useful and efficient matrix for routine surveillance of viral disease.