Immunophenotype and TCR-Vβ repertoire of peripheral blood T-cells in acute infectious mononucleosis
Immunophenotype and TCR-Vβ repertoire of peripheral blood T-cells in acute infectious mononucleosis
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DOI：
10.1016/s1079-9796(03)00014-7
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发表时间：
2003-01-01
影响因子：
2.3
通讯作者：
Justiça, B
中科院分区：
文献类型：
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作者：
Lima, M;Teixeira, MD;Justiça, B
尽管已经发表了一些关于T细胞活化后表型变化的研究，但T淋巴细胞的特异性免疫表型特征以及TCR可变区(TCR-V)限制性T细胞在急性病毒感染期间“体内”扩张的频率仍有待确定。本文报道28例急性传染性单核细胞增多症患者外周血T细胞的免疫表型和TCR-V谱。免疫表型研究采用流式细胞术，使用直接免疫荧光技术和染色-然后裂解样品制备方案，针对一大组T和NK细胞相关市场、激活和黏附相关分子以及TCR-Vβ、-VGamma和-VDelta家族的三色和四色单抗组合进行免疫表型研究。几乎所有患者(27/28)都表现出CD8(+)/TCRalphabeta(+)T细胞的大量增殖，大多数(>；CD2(+高)、CD7(+高)、CD11a(+高)、CD38(+高)、HLA-DR+高、CD28(+/-低)、CD45RO(+高)、CD45RA(-/+低)、CD11b(-/+低)、CD11c(+/-低)、CD16(-)、CD56(-)、CD57(-)、CD62L(-)、CD94(-)、CD158a(-)、CD161(-)、NKB1(-)。此外，与正常人相比，CD3和CD5的水平都略有下降。在CD8(+)/rCRalphabeta(+)T细胞中发现了CD57等晚期激活抗原。在所研究的28例患者中，分别有17例、16例和13例患者的CD4(+)/TCRAlphabeta(+)T细胞、TCRGammadelta(+)T细胞和NK细胞数量增加。CD4(+)/rCRalphabeta(+)和TCRGammadelta(+)T细胞激活的证据依赖于与CD8(+)/TCRalphabeta(+)类似的变化，尽管不太明显，但CD5和CD28水平较高，在CD4+/TCRalphabeta(+)T细胞上CD11c没有反应，而TCRGammadelta(+)T细胞上CD161和CD94水平较高。在研究的14例患者中，12例发现一个或多个TCR-Vbeta家族的小范围扩张，占CD8(+)/TCRalphabeta(+)或CWITCRap‘T细胞室的12+/-7%，而在2例TCRGammadelta(+)T细胞扩增的个体中，检测的TCR-VGamma和-VDelta谱系的分布与对照组相似。本文提出的结果为急性病毒感染期间T细胞的广泛激活提供了证据，并建立了与这种情况相关的免疫表型模式。(C)2003年埃尔塞维尔科学公司(美国)。版权所有。
Although a number of studies on the phenotypic changes that occur after T-cell activation have already been published, the specific immunophenotypic features of T-lymphocytes and the frequency at which TCR-variable region (TCR-V) restricted T-cell expansions occur "in vivo" during acute viral infection still remains to be established. We report on the immunophenotype and TCR-V repertoire of peripheral blood T-cells from 28 patients with acute infectious mononucleosis. Immunophenotypic studies were performed by flow cytometry using direct immunofluorescence techniques and stain-and-then-lyse sample preparation protocols with three- and four-colour combinations of monoclonal antibodies directed against a large panel of T- and NK-cell associated markets, activation- and adhesion-related molecules and TCR-Vbeta, -Vgamma and -Vdelta families. Nearly all patients (27/28) showed a massive expansion of CD8(+)/TCRalphabeta(+) T cells, the majority (>90%) of which displayed an immunophenotype compatible with T-cell activation: CD2(+high) CD7(+low), CD11a(+high), CD38(+high), HLA-DR+high, CD28(+/-low), CD45RO(+high), CD45RA(-/+low), CD11b(-/+low), CD11c(+/-low), CD16(-), CD56(-), CD57(-),CD62L(-),CD94(-),CD158a(-),CD161(-),NKB1(-). Additionally, the levels of both CD3 and CD5 were slightly decreased compared to those found in normal individuals. Late-activation antigens, such as CD57, were found in small proportions of CD8(+)/rCRalphabeta(+) T-cells. Increased numbers of CD4(+)/TCRalphabeta(+) T-cells, TCRgammadelta(+) T-cells and NK-cells were also noticed in 17, 16 and 13 of the 28 cases studied, respectively. Evidence for activation of CD4(+)/rCRalphabeta(+) and TCRgammadelta(+) T-cells relied on changes similar to those described for CD8(+)/TCRalphabeta(+) although less pronounced, except for higher levels of both CD5 and CD28 in the absence of reactivity for CD11c on CD4+/TCRalphabeta(+) T-cells and higher levels of CD161 and CD94 on TCRgammadelta(+) T-cells. Small expansions of one or more TCR-Vbeta families accounting for 12 +/- 7% of either the CD8(+)/TCRalphabeta(+) or the CWITCRap' T-cell compartment were found in 12 of 14 patients studied, whereas the distribution of the TCR-Vgamma and -Vdelta repertoires tested in 2 of the individuals with expanded TCRgammadelta(+) T-cells was similar to that observed in control individuals. The results presented here provide evidence for an extensive T-cell activation during acute viral infection and establish the immunophenotype patterns associated with this condition. (C) 2003 Elsevier Science (USA). All rights reserved.