Effect of Rj Genotype and Cultivation Temperature on the Community Structure of Soybean-Nodulating Bradyrhizobia.
Effect of Rj Genotype and Cultivation Temperature on the Community Structure of Soybean-Nodulating Bradyrhizobia.
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Rj 基因型和培养温度对大豆结瘤慢生根瘤菌群落结构的影响。
DOI：
10.1128/aem.06239-11
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发表时间：
2012
影响因子：
4.4
通讯作者：
他
中科院分区：
文献类型：
--
作者：
S. Shiro Y. Saeki;他
使用来自 Andosol 的 780 个慢根瘤菌 DNA 样本和 13 个四种 Rj 基因型大豆品种，在 33/28°C、28/23°C 和 23/18°C 培养温度 16/8 小时（昼夜度数、小时）下，研究了 Rj 基因型大豆品种上本地慢根瘤菌的结瘤趋势和群落结构。 （非 Rj、Rj2Rj3、Rj4 和 Rj2Rj3Rj4）。基于16S-23S rRNA基因内转录间隔区的PCR产物(PCR-RFLP)的限制性片段长度多态性构建树状图。使用美国农业部的 11 个慢生根瘤菌菌株作为参考。树状图根据参考菌株之间的相似性指示了七个簇。 Bj123簇的占有率随着培养温度的升高而降低，而Bj110簇、Be76簇和Be94簇的占有率随着培养温度的升高而增加。特别地，与其他Rj基因型大豆品种相比，无论培养温度如何升高，Rj2Rj3Rj4基因型大豆都被许多Bj110簇感染。 β多样性与γ多样性的比值（H′β/H′γ）代表了同一大豆品种内不同栽培温度组之间的缓慢根瘤菌群落的差异，表明不同栽培温度下缓慢根瘤菌群落往往存在差异。多维标度分析表明，寄主大豆基因型和培养温度对Bj110簇和Bj123簇的侵染影响了慢根瘤菌群落。这些结果表明，Rj基因型和培养温度影响大豆结瘤缓生根瘤菌的结瘤趋势和群落结构。
The nodulation tendency and community structure of indigenous bradyrhizobia onRjgenotype soybean cultivars at cultivation temperatures of 33/28°C, 28/23°C, and 23/18°C for 16/8 h (day/night degrees, hours) were investigated using 780 bradyrhizobial DNA samples from an Andosol with 13 soybean cultivars of fourRjgenotypes (non-Rj,Rj2Rj3,Rj4, andRj2Rj3Rj4). A dendrogram was constructed based on restriction fragment length polymorphism of the PCR products (PCR-RFLP) of the 16S-23S rRNA gene internal transcribed spacer region. Eleven Bradyrhizobium U.S. Department of Agriculture strains were used as a reference. The dendrogram indicated seven clusters based on similarities among the reference strains. The occupancy rate of the Bj123 cluster decreased with increasing cultivation temperature, whereas the occupancy rates of the Bj110 cluster, Be76 cluster, and Be94 cluster increased with increasing cultivation temperature. In particular, theRj2Rj3Rj4genotype soybeans were infected with a number of Bj110 clusters, regardless of the increasing cultivation temperature, compared to otherRjgenotype soybean cultivars. The ratio of beta diversity to gamma diversity (H′β/H′γ), which represents differences in the bradyrhizobial communities by pairwise comparison among cultivation temperature sets within the same soybean cultivar, indicated that the bradyrhizobial communities tended to be different among cultivation temperatures. Multidimensional scaling analysis indicated that the infection of the Bj110 cluster and the Bj123 cluster by host soybean genotype and the cultivation temperature affected the bradyrhizobial communities. These results suggested that theRjgenotypes and cultivation temperatures affected the nodulation tendency and community structures of soybean-nodulating bradyrhizobia.