Droplet-Scale Estrogen Assays in Breast Tissue, Blood, and Serum
Droplet-Scale Estrogen Assays in Breast Tissue, Blood, and Serum
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DOI：
10.1126/scitranslmed.3000105
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发表时间：
2009-10-07
影响因子：
17.1
通讯作者：
Casper, Robert F.
中科院分区：
文献类型：
--
作者：
Mousa, Noha A.;Jebrail, Mais J.;Casper, Robert F.
雌激素是人类生殖生理学中的关键激素，控制排卵和第二性征。此外，它在乳腺癌的发病机制中起着重要作用。事实上，雌激素受体拮抗剂和芳香酶抑制剂（阻断雌激素生物合成）是用于治疗和预防高危人群的主要药物。尽管其重要性，雌激素的组织浓度并没有常规测量，因为常规技术需要大量的活检样本进行分析。为了满足这一需求，我们开发了一种数字微流体方法，并将其应用于提取和定量1微升乳腺组织匀浆样品（如用细针抽吸收集的）以及全血和血清中的雌激素。该方法可广泛适用于需要频繁分析临床样品中激素的情况（例如，不孕症和癌症）。
Estrogen is a key hormone in human reproductive physiology, controlling ovulation and secondary sexual characteristics. In addition, it plays an important role in the pathogenesis of breast cancer. Indeed, estrogen receptor antagonists and aromatase inhibitors (which block estrogen biosynthesis) are primary drugs used for treatment and prevention in at-risk populations. Despite its importance, tissue concentrations of estrogen are not routinely measured because conventional techniques require large samples of biopsies for analysis. In response to this need, we have developed a digital microfluidic method and applied it to the extraction and quantification of estrogen in 1-microliter samples of breast tissue homogenate (as would be collected with fine-needle aspiration), as well as in whole blood and serum. This method may be broadly applicable to conditions requiring frequent analysis of hormones in clinical samples (for example, infertility and cancer).