Inhibitory action of eugenol compounds on the production of nitric oxide in RAW264.7 macrophages
Inhibitory action of eugenol compounds on the production of nitric oxide in RAW264.7 macrophages
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DOI：
10.2220/biomedres.27.69
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发表时间：
2006-04-01
影响因子：
1.2
通讯作者：
Yoshino, Masataka
中科院分区：
文献类型：
--
作者：
Li, Weihua;Tsubouchi, Ryoko;Yoshino, Masataka
分析丁香酚类化合物对RAW264.7巨噬细胞一氧化氮（NO）生成的影响及其抗炎作用。丁香酚和异丁香酚抑制脂多糖（LPS）依赖性NO的产生，这是由于抑制了诱导型一氧化氮合酶（iNOS）的蛋白质合成。异丁香酚的抑制效果最好，丁香酚的抑制效果较差。异丁香酚对lps依赖性环氧化酶-2 （COX-2）蛋白的表达也有显著抑制作用，而丁香酚对环氧合酶-2蛋白的抑制作用较弱。丁香酚化合物的抗炎作用可能是通过抑制促炎介质NO的产生和COX-2的表达来解释的。
Effects of eugenol compounds on the production of nitric oxide (NO) in RAW264.7 macrophages were analyzed in relation to the anti-inflammatory action of these compounds. Eugenol and isoeugenol inhibited lipopolysaccharide (LPS)-dependent production of NO, which was due to the inhibition of protein synthesis of inducible nitric oxide synthase (iNOS). Isoeugenol showed the most effective inhibitory effect and eugenol was less effective. LPS-dependent expression of cyclooxygenase-2 (COX-2) protein was also inhibited markedly by isoeugenol, and less effectively by eugenol. Anti-inflammatory action of eugenol compounds may be explained by the inhibition of NO production and COX-2 expression, the pro-inflammatory mediators.