FLUORESCENCE MICROSCOPY - REDUCED PHOTOBLEACHING OF RHODAMINE AND FLUORESCEIN PROTEIN CONJUGATES BY NORMAL-PROPYL GALLATE
FLUORESCENCE MICROSCOPY - REDUCED PHOTOBLEACHING OF RHODAMINE AND FLUORESCEIN PROTEIN CONJUGATES BY NORMAL-PROPYL GALLATE
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DOI：
10.1126/science.7112126
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发表时间：
1982-01-01
期刊：
影响因子：
56.9
通讯作者：
SEDAT, JW
中科院分区：
文献类型：
--
作者：
GILOH, H;SEDAT, JW
没食子酸正丙酯（0.1-0.25 M，在甘油中）将用四甲基罗丹明或荧光素缀合抗体标记的细胞结构的荧光褪色速率降低10倍。在荧光显微镜中，免疫荧光标记的[果蝇]细胞的长时间摄影曝光产生具有增加的灵敏度的图像，使得可行的多个数据收集，如连续光学切片。
n-Propyl gallate (0.1-0.25 M, in glycerol) reduces by a factor of 10 the rate of fading of fluorescence of cell structures labeled with tetramethylrhodamine or fluorescein-conjugated antibodies. Prolonged photographic exposure of immunofluorescently labeled [Drosophila] cells in the fluorescence microscope yields images with increased sensitivity, making feasible multiple data collection, as with serial optical sectioning.