Differentiation of embryonic stem cells is induced by GATA factors
Differentiation of embryonic stem cells is induced by GATA factors
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DOI：
10.1101/gad.968802
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发表时间：
2002-04-01
影响因子：
10.5
通讯作者：
Niwa, H
中科院分区：
文献类型：
--
作者：
Fujikura, J;Yamato, E;Niwa, H
胚外内胚层（Extrembryonic endoderm，ExE）是由囊胚期晚期胚胎的内细胞团分化形成的脏层和壁层内胚层，它们在早期胚胎发生中起着重要作用。加塔-6和加塔-4对内脏内胚层分化的重要作用已通过基因敲除小鼠的分析鉴定。在这里，我们报告说，强制表达的加塔-6或加塔-4在胚胎干细胞（ES）细胞是足以诱导适当的分化程序向ExE。我们相信，这是第一个报告的生理分化事件诱导的转录因子在ES细胞的异位表达。
Extraembryonic endoderm (ExE) is differentiated from the inner cell mass of the late blastocyst-stage embryo to form visceral and parietal endoderm, both of which have an important role in early embryogenesis. The essential roles of Gata-6 and Gata-4 on differentiation of visceral endoderm have been identified by analyses of knockout mice. Here we report that forced expression of either Gata-6 or Gata-4 in embryonic stem (ES) cells is sufficient to induce the proper differentiation program towards ExE. We believe that this is the first report of a physiological differentiation event induced by the ectopic expression of a transcription factor in ES cells.