MARKER OF PERIPHERAL-BLOOD GRANULOCYTES AND MONOCYTES OF MAN RECOGNIZED BY 2 MONOCLONAL-ANTIBODIES VEP8 AND VEP9 INVOLVES THE TRISACCHARIDE 3-FUCOSYL-N-ACETYLLACTOSAMINE
MARKER OF PERIPHERAL-BLOOD GRANULOCYTES AND MONOCYTES OF MAN RECOGNIZED BY 2 MONOCLONAL-ANTIBODIES VEP8 AND VEP9 INVOLVES THE TRISACCHARIDE 3-FUCOSYL-N-ACETYLLACTOSAMINE
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DOI：
10.1002/eji.1830130407
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发表时间：
1983-01-01
影响因子：
5.4
通讯作者：
FEIZI, T
中科院分区：
文献类型：
--
作者：
GOOI, HC;THORPE, SJ;FEIZI, T
针对[人]早幼粒单核细胞白血病细胞系HL 60产生的两种杂交瘤抗体（VEP 8和VEP 9）先前已显示出将人粒细胞和单核细胞与外周血的其它细胞区分开。两种抗体均识别具有以下序列的碳水化合物结构3-岩藻糖基-N-乙酰基乳糖胺：图形 **。该结构与识别小鼠早期胚胎抗原的抗小鼠畸胎瘤细胞杂交瘤抗体（抗SSEA-1）所识别的结构相同。直到最近，这种碳水化合物结构在糖蛋白和鞘糖脂中被认为是罕见的。通过化学和免疫化学方法检测到该序列的人类和动物糖蛋白越来越多。调查了这些信息，并讨论了这种和其他碳水化合物结构如何作为分化或肿瘤相关抗原。
Two hybridoma antibodies (VEP8 and VEP9) raised against the [human] promyelomonocytic leukemia cell line HL60 have previously been shown to distinguish human granulocytes and monocytes from other cells of the peripheral blood. Both antibodies recognize the carbohydrate structure 3-fucosyl-N-acetyllactosamine with the following sequence: .**GRAPHIC**. This structure is the same as that recognized by a hybridoma antibody against mouse teratocarcinoma cells (anti-SSEA-1) which recognizes an early embryonic antigen in the mouse. Until recently this carbohydrate structure was considered rare among glycoproteins and glycosphingolipids. There is a growing list of human and animal glycoproteins in which this sequence was detected by chemical and immunochemical methods. This information was surveyed and how this and other carbohydrate structures behave as differentiation- or tumor-associated antigens was discussed.