A plea for appraisal and appreciation of immunohistochemistry in the assessment of prognostic and predictive markers in invasive breast cancer.
A plea for appraisal and appreciation of immunohistochemistry in the assessment of prognostic and predictive markers in invasive breast cancer.
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呼吁对免疫组织化学在侵袭性乳腺癌预后和预测标记物评估中的评估和评价。
DOI：
10.1016/j.breast.2017.10.012
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发表时间：
2018
期刊：
影响因子：
3.9
通讯作者：
L. Libbrecht
中科院分区：
文献类型：
--
作者：
M. V. van Bockstal;G. Floris;C. Galant;K. Lambein;L. Libbrecht
这一观点是对免疫组化在当前乳腺癌诊断中的价值和优点的个人思考。免疫组化是浸润性乳腺癌分子分型的主要方法。激素受体状态和HER2表达的免疫组织化学评估用于确定乳腺癌内在亚型的临床病理学替代物，其指导新辅助和辅助治疗。基因组预后特征和基于mRNA的定性分析的出现使一些临床医生和研究人员怀疑是否应该放弃免疫组化。然而，这些基于mRNA的测试的危险和陷阱不容忽视。该观点简要概述了免疫组化和qPCR的质量问题，并简要总结了免疫组化和基于mRNA的检测在浸润性乳腺癌预后和预测标志物中的相关性的现有证据。我们强烈提倡使用免疫组织化学，因为它将有价值的空间信息与蛋白质表达的量化相结合。
This viewpoint is a personal reflection on the values and merits of immunohistochemistry in current breast cancer diagnosis. Immunohistochemistry is a validated mainstay in molecular subtyping of invasive breast cancer. Immunohistochemical assessment of hormone receptor status and HER2 expression is used to determine the clinico-pathological surrogate of breast cancer intrinsic subtypes, which guide neoadjuvant and adjuvant therapy. The advent of genomic prognostic signatures and qualitative mRNA-based assays makes some clinicians and researchers wonder whether immunohistochemistry should be abandoned. However, the perils and pitfalls of these mRNA-based tests cannot be neglected. This viewpoint offers a brief overview of quality issues in immunohistochemistry and qPCR, as well as a concise summary of currently available evidence on the correlation of immunohistochemistry and mRNA-based testing for prognostic and predictive markers in invasive breast cancer. We strongly advocate the use of immunohistochemistry as it integrates valuable spatial information with quantification of protein expression.
影响因子：
4.6
作者：
M. E. Hammond;Daniel F. Hayes;M. Dowsett;D. C. Allred;K. Hagerty;S. Badve;P. Fitzgibbons;G. Francis
通讯作者：
M. E. Hammond;Daniel F. Hayes;M. Dowsett;D. C. Allred;K. Hagerty;S. Badve;P. Fitzgibbons;G. Francis