Measurement of histone mRNA transcript abundance in Xenopus oocytes by a quantitative primer extension assay.
Measurement of histone mRNA transcript abundance in Xenopus oocytes by a quantitative primer extension assay.
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通过定量引物延伸测定测量非洲爪蟾卵母细胞中的组蛋白 mRNA 转录本丰度。
DOI：
10.1002/mrd.1080250105
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发表时间：
1990
影响因子：
2.5
通讯作者：
Perry,M
中科院分区：
文献类型：
--
作者：
Brashears-Macatee,S;Hinkley,C;Perry,M
采用定量引物延伸法测定了非洲爪蟾成熟卵母细胞中组蛋白基因转录本的质量，该方法使用大量过量的基因特异性寡核苷酸引物，并连续掺入放射性标记的脱氧核苷三磷酸前体，比使用末端标记引物的引物延伸法更灵敏和定量。在成熟的异种卵母细胞中，5种主要组蛋白基因的每一种都有大约2 × 108个拷贝的mRNA的化学计量学量。这些观察结果与两栖动物卵子发生过程中这些基因的转录以细胞周期非依赖性方式协调调节的模型一致。
A quantitative primer extension method was used to measure the mass of histone gene transcripts in mature oocytes of the amphibianXenopus laevis.The procedure, using a large excess of gene‐specific oligonucleotide primer and continuous incorporation of a radiolabeled deoxynucleoside triphosphate precursor, is more sensitive and quantitative than primer extension assays employing end‐labeled primers. It was determined that there are stoichometric amounts, approximately 2 × 108copies, of mRNA for each of the five major histone gene classes in matureXenopusoocytes. These observations are consistent with a model whereby transcription of these genes is coordinately regulated in a cell cycle‐independent manner during amphibian oogenesis.