A new role for histone demethylases in the maintenance of plant genome integrity.
A new role for histone demethylases in the maintenance of plant genome integrity.
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DOI：
10.7554/elife.58533
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发表时间：
2020-10-27
期刊：
影响因子：
7.7
通讯作者：
Gutierrez-Marcos J
中科院分区：
文献类型：
--
作者：
Antunez-Sanchez J;Naish M;Ramirez-Prado JS;Ohno S;Huang Y;Dawson A;Opassathian K;Manza-Mianza D;Ariel F;Raynaud C;Wibowo A;Daron J;Ueda M;Latrasse D;Slotkin RK;Weigel D;Benhamed M;Gutierrez-Marcos J
由Polycomb抑制复合物2（PRC2）沉积的组蛋白修饰在大多数多细胞真核生物的生长、发育和适应环境波动的控制中起着关键作用。PRC2的催化活性被Jumonji型（JMJ）组蛋白脱甲基酶抵消，从而形成H3K27me3的基因组分布。在这里，我们表明，两个JMJ组蛋白去甲基化酶在拟南芥，早开花6（ELF 6）和早开花6（REF 6）的关系，发挥不同的作用，在H3K27me3和H3K27me1的稳态。我们发现，在有性生殖过程中未能重置这些染色质标记导致组蛋白标记的跨代遗传，这会导致异染色质基因座DNA甲基化的丧失和转座子激活。因此，Jumonji型组蛋白去甲基化酶在植物中发挥双重作用，通过发育帮助维持转录状态，并在有性生殖期间保护基因组完整性。
Histone modifications deposited by the Polycomb repressive complex 2 (PRC2) play a critical role in the control of growth, development, and adaptation to environmental fluctuations of most multicellular eukaryotes. The catalytic activity of PRC2 is counteracted by Jumonji-type (JMJ) histone demethylases, which shapes the genomic distribution of H3K27me3. Here, we show that two JMJ histone demethylases in Arabidopsis, EARLY FLOWERING 6 (ELF6) and RELATIVE OF EARLY FLOWERING 6 (REF6), play distinct roles in H3K27me3 and H3K27me1 homeostasis. We show that failure to reset these chromatin marks during sexual reproduction results in the transgenerational inheritance of histone marks, which cause a loss of DNA methylation at heterochromatic loci and transposon activation. Thus, Jumonji-type histone demethylases play a dual role in plants by helping to maintain transcriptional states through development and safeguard genome integrity during sexual reproduction.