Improved instrumentation for blood flow velocity measurements in the microcirculation of small animals
Improved instrumentation for blood flow velocity measurements in the microcirculation of small animals
复制标题
DOI：
10.1063/1.2668504
复制
发表时间：
2007-02-01
影响因子：
1.6
通讯作者：
de Melo, Pedro Lopes
中科院分区：
文献类型：
--
作者：
de Mesquita, Jayme Alves, Jr.;Bouskela, Eliete;de Melo, Pedro Lopes
微循环是内径小于100 μ m的血管循环系统的总称，其主要功能是组织营养和供氧。在微循环研究中，了解血液中氧合血红蛋白的含量及其移动速度是很重要的。目前的工作描述了一种经典的基于硬件的仪器的改进，该仪器通常用于监测小动物微循环中的血流速度。它由一个虚拟仪器组成，可以很容易地集成到现有的基于硬件的系统中，有助于减少操作员相关的偏差，并允许数字处理和存储。介绍了改进后的仪器的设计和校准，以及分别用电模型和小动物获得的体外和体内结果。体内研究结果表明，与动脉和小动脉相比，这种新系统能够检测到血流速度的小幅下降（p < 0.002），并进一步降低毛细血管（p < 0.0001）。与毛细血管和小静脉（p < 0.001）以及小静脉和静脉（p < 0.001）相比，流速显著增加。这些结果与生物物理原理非常吻合。此外，该设备的改进使我们能够清楚地观察到药物干预引起的血流变化，这表明该系统具有足够的时间分辨率来跟踪这些微循环事件。这些结果也与生理学密切一致，证实了改进系统的高科学潜力，并表明该仪器也可用于药理学评估。(c) 2007年美国物理学会。
Microcirculation is the generic name of vessels with internal diameter less than 100 mu m of the circulatory system, whose main functions are tissue nutrition and oxygen supply. In microcirculatory studies, it is important to know the amount of oxyhemoglobin present in the blood and how fast it is moving. The present work describes improvements introduced in a classical hardware-based instrument that has usually been used to monitor blood flow velocity in the microcirculation of small animals. It consists of a virtual instrument that can be easily incorporated into existing hardware-based systems, contributing to reduce operator related biases and allowing digital processing and storage. The design and calibration of the modified instrument are described as well as in vitro and in vivo results obtained with electrical models and small animals, respectively. Results obtained in in vivo studies showed that this new system is able to detect a small reduction in blood flow velocity comparing arteries and arterioles (p < 0.002) and a further reduction in capillaries (p < 0.0001). A significant increase in velocity comparing capillaries and venules (p < 0.001) and venules and veins (p < 0.001) was also observed. These results are in close agreement with biophysical principles. Moreover, the improvements introduced in the device allowed us to clearly observe changes in blood flow introduced by a pharmacological intervention, suggesting that the system has enough temporal resolution to track these microcirculatory events. These results were also in close conformity to physiology, confirming the high scientific potential of the modified system and indicating that this instrument can also be useful for pharmacological evaluations. (c) 2007 American Institute of Physics.