Distribution patterns of Wolbachia endosymbionts in the closely related flower bugs of the genus Orius: implications for coevolution and horizontal transfer.
Distribution patterns of Wolbachia endosymbionts in the closely related flower bugs of the genus Orius: implications for coevolution and horizontal transfer.
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沃尔巴克氏体内共生体在 Orius 属密切相关的花虫中的分布模式：对协同进化和水平转移的影响。
DOI：
10.1007/s00248-012-0042-x
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发表时间：
2012
影响因子：
3.6
通讯作者：
Stouthamer R
中科院分区：
文献类型：
--
作者：
Watanabe M;Tagami Y;Miura K;Kageyama D;Stouthamer R
垂直传播是内共生体沃尔巴克氏体在无脊椎动物宿主中传播的主要途径，但不同宿主之间的水平传播被认为发生过多次。然而，目前还不清楚沃尔巴克氏体如何在密切相关的宿主中传播。我们专注于属于Orius属的微小海盗虫的密切相关物种，它们是农作物中重要的生物控制剂，因为它们是各种微小害虫（如蓟马）最有用的天敌。在这里，我们检查了五种小花蝽（小花蝽，长井小花蝽，小花蝽，小花蝽strigicollis，和小花蝽tantillus）从日本的8个地理位置的沃尔巴克氏体感染。根据Wolbachiasurface protein（wsp）基因序列分析，发现两株不同的菌株wOus 1和wOus 2。此外，多位点序列分型显示，每种菌株都包含两种在单个核苷酸上不同的变体。两株Wolbachia菌株在不同宿主中的分布模式不同：在O. strigicollis;在O. nagaii;在O. sauteri;单次感染wOus 1在O.结果表明，在单个群体中，除个别个体外，其余个体均为阴性;坦蒂勒斯Wolbachiamay分布格局的这种差异可能反映了Wolbachiamay感染Orius物种的进化历史和/或Orius物种之间的生态和生理差异，这些差异决定了两个Wolbachiamay菌株的入侵和维持。
Vertical transmission is the primary route of the endosymbiontWolbachiafor its own spread among invertebrate hosts, but horizontal transmission between different hosts is believed to have occurred multiple times. However, it is not well known howWolbachiacommonly spread among closely related hosts. We focused on the closely related species of the minute pirate bugs belonging to the genusOrius, which are important biological control agents in agricultural crops because they are the most useful natural enemy of various tiny pests, such as thrips. Here, we examined fiveOriusspecies (Orius sauteri,Orius nagaii,Orius minutus,Orius strigicollis, andOrius tantillus) from eight geographic localities in Japan forWolbachiainfection. Two distinct strains,wOus1 andwOus2, were detected based onWolbachiasurface protein (wsp) gene sequencing. Furthermore, multilocus sequence typing revealed that each of the strains comprised two variants that differed in a single nucleotide. The overall distribution patterns of the twoWolbachiastrains were found to differ among host species: prevalent double infection withwOus1 andwOus2 inO. strigicollis; fixation of single infection withwOus2 inO. nagaii; occurrence of single infection withwOus1 inO. sauteri; prevalence of single infection withwOus1 inO. minutuswith an exception in a single population; and lack ofWolbachiainfection inO. tantillus. Such differences in the distribution patterns ofWolbachiamay reflect the evolutionary history ofWolbachiainfection amongOriusspecies and/or ecological and physiological differences among theOriusspecies that determine the invasiveness and maintenance of the twoWolbachiastrains.