Membrane Nanotubes in the Electric Field As a Model for Measurement of Mechanical Parameters of the Lipid Bilayer
Membrane Nanotubes in the Electric Field As a Model for Measurement of Mechanical Parameters of the Lipid Bilayer
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DOI：
10.1134/s1990747807020110
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发表时间：
2007-06-01
影响因子：
0.5
通讯作者：
Bashkirov, P. V.
中科院分区：
文献类型：
--
作者：
Bashkirov, P. V.
从保持在高侧向张力下的平面双层类脂膜中拉出膜圆柱管，可得到内径为几纳米的纳米管(NT)。当NT被拉入电解液中时，它的内部是导电的，由NT的电导-长度关系的双曲线拟合计算出其内径。根据膜脂组成的不同，NT的内径从2 nm到20 nm不等，胆固醇和溶质脂含量越高，NT管越宽。在NT膜上施加电场可以根据李普曼效应改变有效侧向张力。在沿NT施加不同电压的情况下，测量了NT半径，并假设NT的形状仅略微偏离圆柱形，重新计算了膜的弯曲模数。本文得到的磷脂膜刚性计算值与以前发表的数据相一致。
Pulling a membrane cylindrical tubule from a planar bilayer lipid membrane held under a high lateral tension produces a nanotube (NT) with an internal radius of several nanometers. When NT is pulled in an electrolyte solution, its interior is conductive and the internal radius is calculated from the hyperbolic fitting of the NT conductance-length relationship. Depending on the membrane lipid composition, the internal radius of NT varies from 2 to 20 nm, the higher the cholesterol and lysolipid content, the wider the tubes. The application of an electrical field across the NT membrane allows variation of effective lateral tension according to the Lippman effect. The NT radii were measured at different values of voltage applied along NT, and the membrane bending modulus was recalculated upon the supposition that the shape of NT deviates from cylindrical only slightly. The calculated values of the phospholipid membrane stiffness obtained in this work correspond to previously published data.