Increased PC-1 phosphodiesterase activity and inhibition of glucose uptake in adipocytes of type 2 diabetic rats
Increased PC-1 phosphodiesterase activity and inhibition of glucose uptake in adipocytes of type 2 diabetic rats
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DOI：
10.1007/s11010-006-0387-x
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发表时间：
2006-12-01
影响因子：
4.3
通讯作者：
Ragoobirsingh, Dalip
中科院分区：
文献类型：
--
作者：
Barrett, Karlene;McGrowder, Donovan;Ragoobirsingh, Dalip
本研究旨在了解2型糖尿病动物模型中胰岛素刺激信号转导通路缺陷的细胞机制。我们研究了在体外PC-1磷酸二酯酶活性和葡萄糖摄取的脂肪组织链脲佐菌素（STZ）诱导的2型糖尿病大鼠。与对照组（0.29 +/-0.05 nmol PNTP水解/mg蛋白/min，p < 0.05）相比，糖尿病大鼠脂肪组织中的PC-1活性显着增加（0.54 +/-0.08 nmol PNTP水解/mg蛋白/min）。在胰岛素刺激（100 nM）后，对照组脂肪组织中的葡萄糖摄取（4.17 +/- 1.28 x 10（-8）μ mol/mg/min）显著高于糖尿病大鼠（1.26 +/- 0.35 x 10（-8）; p < 0.05）。这些结果表明，PC-1磷酸二酯酶活性升高和脂肪组织中葡萄糖摄取减少可能是2型糖尿病发展的后天特征。
This study was designed to understand the cellular mechanisms responsible for defects in the insulin-stimulated signal transduction pathway in a type 2 diabetic animal model. We examined the in vitro PC-1 phosphodiesterase activity and glucose uptake in adipose tissue of streptozotocin (STZ)-induced type 2 diabetic rats. The PC-1 activity was significantly increased in adipose tissue of diabetic rats (0.54 +/- 0.08 nmol PNTP hydrolyzed/mg protein/min) compared with controls (0.29 +/- 0.05 nmol PNTP hydrolyzed/mg protein/min, p < 0.05). Upon insulin stimulation (100 nM), glucose uptake in the adipose tissue of the controls (4.17 +/- 1.28 x 10(-8) mu mol/mg/min) was significantly higher than that in the diabetic rats (1.26 +/- 0.35 x 10(-8); p < 0.05). These results suggest that elevated PC-1 phosphodiesterase activity and decreased glucose uptake in adipose tissues may be acquired characteristics contributing to the development of type 2 diabetes mellitus.