Intramembrane Proteins Clustering Induced by 50 Hz Pulsed Magnetic Fields is Reverted to a Random Distribution in the Absence of the Field Exposure
Intramembrane Proteins Clustering Induced by 50 Hz Pulsed Magnetic Fields is Reverted to a Random Distribution in the Absence of the Field Exposure
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DOI：
10.1007/978-1-4615-4867-6_136
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
F. Marinelli;F. Bersani;S. Santi;M. Riccio;S. Petrini;A. Valmori;N. Maraldi
中科院分区：
文献类型：
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作者：
F. Marinelli;F. Bersani;S. Santi;M. Riccio;S. Petrini;A. Valmori;N. Maraldi
极低频电磁场（ELF）的生物效应似乎是由质膜介导的。已经提出了许多假说来解释ELFEMF影响细胞功能的机制。特别地，已经提出EMF可能影响细胞受体功能，或离子通道形成或受体封端。(Adey和Lawrence 1984; Chiabrera等1991）在某些条件下，据报道电渗和电泳效应影响膜内蛋白（IMP）分布（Poo和罗宾逊1977，Mc Laughlin和Poo 1981; Giugni等1987）。IMP通常随机分布在细胞膜上，簇集是与细胞的特异性和非特异性刺激相关的事件（Brown和Loew 1994）。在以前的工作中（Bersani等，1997年），我们发现，一个显着的集群IMP发生后1-2小时暴露于50赫兹脉冲磁场（PMF）。这项工作的目的是验证这种影响是否是可逆的。为此目的，NIH 3 T3成纤维细胞暴露于PMF一段时间能够诱导最大的IMP聚集，维持另一段时间不暴露于该领域，以确定是否IMP分布可以恢复到对照条件。
Biological effects of extremely low frequency (ELF) electromagnetic fields (EMF) seem to be mediated by the plasma membrane. Many hypotheses have been suggested to explain the mechanisms by which ELFEMF affect cell functions. In particular it has been proposed that EMF may affect the cell receptor function, or ion channel formation or receptor capping. (Adey and Lawrence 1984; Chiabrera et al. 1991) In some conditions electroosmotic and electrophoretic effects have been reported to affect the intramembrane protein (IMP) distribution (Poo and Robinson 1977, Mc Laughlin and Poo 1981; Giugni et al. 1987). The IMP are normally randomly distributed at the cell membrane and clusterization is an event related to specific and aspecific stimulations of the cell (Brown and Loew 1994). In a previous work (Bersani et al. 1997) we found that a significant clusterization of IMP takes place after 1–2 hours of exposure to 50 Hz pulsed magnetic fields (PMF). The aim of this work was to verify whether such effect was reversible or not. For this purpose NIH 3T3 fibroblasts exposed to PMF for a time capable to induce maximal IMP clusterization were maintained for a further period of time not exposed to the field in order to determine whether IMP distribution can be reverted to control conditions.