Decision of spindle poles and division plane by double preprophase bands in a BY-2 cell line expressing GFP-tubulin.
Decision of spindle poles and division plane by double preprophase bands in a BY-2 cell line expressing GFP-tubulin.
复制标题
DOI：
10.1093/pcp/pci055
复制
发表时间：
2005-03
影响因子：
4.9
通讯作者：
A. Yoneda;Minori Akatsuka;H. Hoshino;F. Kumagai;S. Hasezawa
中科院分区：
文献类型：
--
作者：
A. Yoneda;Minori Akatsuka;H. Hoshino;F. Kumagai;S. Hasezawa
微管的前前期带（PPB）被认为参与决定未来的分裂位点。本研究利用转绿色荧光蛋白（GFP）-微管蛋白基因的BY-2细胞，研究了双PPBs对纺锤体形成和胞质分裂方向决定的影响。在前期，大多数细胞与双PPBs形成多极纺锤体，而所有细胞与单PPBs形成正常的双极纺锤体，明确暗示PPB在决定纺锤体极。但在分裂中期，两种细胞都具有双极纺锤体，表明在前中期存在纠正机制。从前中期到中期，双PPB细胞中的纺锤体改变了它们的方向，变得倾斜于细胞伸长轴，并且这些方向在成膜体中保持，并导致倾斜的分裂平面。当肌动蛋白微丝被破坏时，这些倾斜的细胞板减少，并且在双PPBs存在的地方出现双肌动蛋白耗尽区（ADZ）。这些结果表明，适当的胞质分裂所需的信息可以从PPBs转移到ADZ，即使在双PPBs的情况下。
The preprophase band (PPB) of microtubules is thought to be involved in deciding the future division site. In this study, we investigated the effects of double PPBs on spindle formation and the directional decision of cytokinesis by using transgenic BY-2 cells expressing green fluorescent protein (GFP)-tubulin. At prophase, most of the cells with double PPBs formed multipolar spindles, whereas all cells with single PPBs formed normal bipolar spindles, clearly implicating the PPB in deciding the spindle poles. At metaphase, however, both cell types possessed the bipolar spindles, indicating the existence of correctional mechanism(s) at prometaphase. From prometaphase to metaphase, the spindles in double PPB cells altered their directions to become oblique to the cell-elongating axis, and these orientations were maintained in the phragmoplast and resulted in the oblique division planes. These oblique cell plates decreased when actin microfilaments were disrupted, and double actin-depleted zones (ADZs) appeared where the double PPBs had existed. These results suggest that the information necessary for proper cytokinesis may be transferred from the PPBs to the ADZs, even in the case of the double PPBs.