QTL MAPPING FOR EARLY VIGOR RELATED TRAITS IN AN ELITE WHEAT-BREEDING PARENT CHUANMAI 42 DERIVED FROM SYNTHETIC HEXAPLOID WHEAT
QTL MAPPING FOR EARLY VIGOR RELATED TRAITS IN AN ELITE WHEAT-BREEDING PARENT CHUANMAI 42 DERIVED FROM SYNTHETIC HEXAPLOID WHEAT
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合成六倍体小麦优良育种亲本川麦42早期活力相关性状的QTL定位
DOI：
10.21162/pakjas/18.4488
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发表时间：
2018-03-01
影响因子：
0.8
通讯作者：
Yang, Wuyun
中科院分区：
文献类型：
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作者：
Li, Jun;Wan, Hongshen;Yang, Wuyun
幼苗生长是作物可靠生长的重要组成部分，早期活力的提高往往与耐旱性、生物量和产量的提高有关。川麦42是人工合成六倍体小麦的优良育种亲本，在我国西南地区小麦育种中得到了广泛应用。以川麦42为材料，构建了127个重组自交系（RIL），通过QTL定位，剖析了川麦42早熟性强的分子基础，并对全展开叶数、总叶面积、在改良的Hoagland营养液中培养21 d和28 d后，分别测定其地上部鲜重、地上部干重、主根数、根干重和相对生长速率。在含有148个SSR标记和644个DArT标记的连锁图上，共检测到40个QTL，分布在14条染色体上。在解释表型变异10%以上的25个QTL中，有17个QTL通过川麦42个等位基因对相关性状起促进作用。在5A、6A、6 B和7A染色体上共检测到4个QTL簇。其中，川麦42的6 B和7A基因组区域具有增强幼苗早期活力的QTL簇，川农16的6 B和7A基因组区域具有增强幼苗早期活力的QTL簇。6 B染色体上Xgwm 154-Wpt 7846的QTL簇是表型效应最强的重要基因组区域，且在生育期的伸长过程中也得到加强。本研究结果为利用川麦42作育种亲本进行早期活力的分子改良提供了理论依据。
Seedling growth is one essential component of reliable crop establishment, and greater early vigor is often associated with greater drought tolerance, biomass and grain yield Chuanmai 42 derived from synthetic hexaploid wheat has been widely used as an elite wheat-breeding parent in wheat breeding program of Southwest China In this study, 127 recombinant inbred lines (RILs) developed from Chuanmai 42 were used to dissect the molecular basis of its great early vigor by QTL mapping, and seven early vigor related traits including number of all expanded leaves, total leaf area, shoot fresh weight, shoot dry weight, number of the main roots, root dry weight and relative growth rate were evaluated for this RILs after 21-day and 28-day growth under a modified Hoagland nutrient solution, respectively. Based on a linkage map containing 148 S SR and 644 DArT makers, a total of 40 QTLs for these traits were detected on 14 chromosomes. Among the 25 QTLs with the phenotypic variance explained more than 10%, 17 QTLs enhanced the related traits through Chuanmai 42 alleles. A total of 4 genomic regions with QTL clusters were detected on chromosomes 5A, 6A, 6B and 7A, respectively. Among them, the genomic regions with QTL clusters on 6B and 7A from Chuanmai 42 enhanced early vigor of seedling, and the other two were from Chuannong 16. Moreover, QTL cluster in Xgwm154-Wpt7846 of chromosome 6B was the important genomic region with the strongest phenotypic effects that were also strengthened during elongation of the growth period. The findings of this study are relevant to the molecular improvement of early vigor when using Chuanmai 42 as a breeding parent.