Superantigen-induced TCR alpha locus secondary rearrangement: role in tolerance induction.
Superantigen-induced TCR alpha locus secondary rearrangement: role in tolerance induction.
复制标题
超抗原诱导的 TCR α 基因座二次重排：在耐受诱导中的作用。
作者：
Ching‐Yu Huang;R. Golub;Gillian E. Wu;O. Kanagawa
体内超抗原免疫诱导超抗原特异性 T 细胞短暂激活，随后进入超抗原无反应状态。在这项研究中，我们使用TCRα敲入小鼠，其中敲入的α链可以通过二次重排被内源性α链取代，超抗原的免疫改变了外周超抗原特异性T细胞上的TCRα链表达，诱导重组激活基因的表达，并在TCRα链位点产生DNA双链断裂。这些结果表明病毒超抗原能够诱导外周 TCR 修正。因此，我们的研究结果为病原体-免疫系统相互作用提供了新的视角。
Immunization with superantigen in vivo induces transient activation of superantigen-specific T cells, followed by a superantigen-nonresponsive state. In this study, using a TCR alpha knock-in mouse in which the knock-in alpha-chain can be replaced with endogenous alpha-chain through secondary rearrangement, we show that immunization of superantigen changes the TCR alpha-chain expression on peripheral superantigen-specific T cells, induces expression of recombination-activating genes, and generates DNA double-strand breaks at the TCR alpha-chain locus. These results suggest that viral superantigens are capable of inducing peripheral TCR revision. Our findings thus provide a new perspective on pathogen-immune system interaction.