Aedes aegypti vectorial capacity is determined by the infecting genotype of dengue virus
Aedes aegypti vectorial capacity is determined by the infecting genotype of dengue virus
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DOI：
10.4269/ajtmh.2006.75.886
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发表时间：
2006-11-01
影响因子：
3.3
通讯作者：
Rico-Hesse, Rebeca
中科院分区：
文献类型：
--
作者：
Anderson, Justin R.;Rico-Hesse, Rebeca
在过去三十年中，导致严重出血性疾病的登革热病毒在美洲取代了毒性较弱的毒株。美国（AM）基因型登革热血清2型在西半球和南太平洋流行，导致登革热（DF）暴发，但与登革出血热（DHF）无关。东南亚（SEA）登革热基因型于1981年传入本半球，已造成多例登革出血热暴发，并在若干国家取代了AM基因型。我们通过在南德克萨斯州采集的埃及伊蚊感染这两种基因型的6种病毒，研究了病毒基因型对传播潜力的影响。SEA感染的蚊子中肠的病毒复制量显著高于am感染的蚊子，SEA感染的蚊子唾液腺中检测到病毒特异性蛋白的时间比am感染的蚊子早7天。登革热病毒在唾液腺中更早出现，导致这些蚊子传播可引起登革出血热的病毒的能力估计增加了2至65倍。这可能是毒性更强的黄病毒在全球传播和取代其他病毒的机制之一。
Dengue viruses causing severe, hemorrhagic disease have displaced less virulent strains in the Americas during the past three decades. The American (AM) genotype of dengue serotype 2 has been endemic in the Western Hemisphere and South Pacific, causing outbreaks of dengue fever (DF), but has not been linked to dengue hemorrhagic fever (DHF). The Southeast Asian (SEA) genotype of dengue was introduced into this hemisphere in 1981, has caused outbreaks with numerous cases of DHF, and has displaced the AM genotype in several countries. We investigated the effect of viral genotype on the potential for transmission by infecting Aedes aegypti mosquitoes collected in South Texas with six viruses, representing these two genotypes. Viral replication in the midgut was significantly higher in SEA-infected mosquitoes, and virus-specific proteins could be detected in salivary glands 7 days earlier in SEA- than AM-infected mosquitoes. This much earlier appearance of dengue virus in salivary glands resulted in an estimated 2- to 65-fold increase in the vectorial capacity of these mosquitoes for the viruses that can cause DHF. This may be one of the mechanisms through which more virulent flaviviruses spread and displace others globally.