Microelectrode array evaluation of gut pacemaker activity in wild-type and W/Wv mice
Microelectrode array evaluation of gut pacemaker activity in wild-type and W/Wv mice
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DOI：
10.1016/j.bios.2009.06.006
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发表时间：
2009-09-15
影响因子：
12.6
通讯作者：
Hirose, Kenzo
中科院分区：
文献类型：
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作者：
Nakayama, Shinsuke;Ohishi, Ryotaro;Hirose, Kenzo
肌间神经丛区域的Cajal间质细胞(ICC-MYP)形成网络并产生基本的起搏电活动。这一形态特征使我们相信这些细胞可能是胃肠道(GI)运动协调活动所必需的。我们的目的是提出一种新的评价ICC电活动及其网络功能的方法。在细胞外液中加入硝苯地平和河豚毒素(TTX)分别抑制平滑肌细胞和神经元的活动，用8×8微电极阵列(MEA)，极距150微米，同时测量接近1 mm(2)区域的场电位。我们比较了野生型(WT)和W/W-v小鼠回肠肌标本的空间电活动。在时空分析中，WT的基本电活动与传播延迟具有很好的同步性，而W/W-V的基础电活动幅度较小，出现的时间不规律。WT的功率谱有一个与ICC-MYP起搏频率对应的显著峰值，而W/W-v的功率谱缺乏该峰。因此，WT组9.4~27.0cpm的频谱功率比明显大于W/W-V组。综上所述，MEA测量表明，网络形成的ICC-MYP不仅产生并协调基本的电活动。以ICC网络的形态和功能损害为基础的GI运动障碍，范围可达数百微米，可能在未来的广泛研究中被发现。(C)2009爱思唯尔B.V.保留所有权利。
interstitial cells of Cajal in the myenteric plexus region (ICC-MyP) form a network and generate basal pacemaking electrical activity. This morphological feature leads us to believe that these cells may be essential for the coordinating actions of gastrointestinal (GI) motility. We aim to propose a new method for functional assessment of ICC electrical activity and its network. Field potentials in a similar to 1 mm(2) region were simultaneously measured using an 8 x 8 microelectrode array (MEA) with a polar distance of 150 mu m. The extracellular solution contained nifedipine and tetrodotoxin (TTX) to suppress activities of smooth muscle cells and neurons, respectively. We compared spatial electrical activities between ileal muscle preparations from wild-type (WT) and W/W-v mice. In spatio-temporal analyses, basal electrical activities were well synchronized with a propagation delay in WT while those in W/W-v were small in amplitude and irregular in occurrence. The power spectrum in WT had a prominent peak corresponding to the frequency of ICC-MyP pacemaker activity, while that of W/W-v lacked it. Consequently, the ratio of the spectral power in 9.4-27.0 cpm was significantly larger in WT than in W/W-v. In conclusion, MEA measurements demonstrated that the network-forming ICC-MyP not only generates but also coordinates basal electrical activities. Disorders of GI motility based on morphological and functional impairments of ICC network with the range of several hundreds of micrometers, could be uncovered in future extensive studies. (C) 2009 Elsevier B.V. All rights reserved.