Effects of ultrasound on vascular endothelial growth factor in cartilage, synovial fluid, and synovium in rabbit knee osteoarthritis
Effects of ultrasound on vascular endothelial growth factor in cartilage, synovial fluid, and synovium in rabbit knee osteoarthritis
复制标题
超声对兔膝骨关节炎软骨、滑液和滑膜中血管内皮生长因子的影响。
DOI：
10.18388/abp.2020_5238
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发表时间：
2020-01-01
影响因子：
1.7
通讯作者：
Lin, Qiang
中科院分区：
文献类型：
--
作者：
Luo, Qinglu;Fan, Siqin;Lin, Qiang
超声常用于治疗膝关节骨关节炎（KOA），在缓解疼痛和炎症以及延缓软骨退变方面具有独特的优势，但其潜在机制尚不清楚。本研究旨在探讨超声对兔膝关节骨性关节炎模型软骨、滑膜和滑液中血管内皮生长因子（VEGF）表达的治疗作用。24只新西兰大白兔随机分为超声组（A组）、假超声组（B组）和无ACLT对照组（C组）。前交叉韧带切断（ACLT）后6周，A组接受超声治疗，B组接受假超声治疗。2周后观察滑膜和软骨的形态学变化。分别使用Mankin量表和Krenn V评分对软骨和滑膜进行评分。通过免疫组化、免疫印迹和RT-PCR分析ACLT膝关节软骨、SF和滑膜中VEGF的表达。B组软骨退变和滑膜炎最严重，C组最轻。同样，Mankin评分和Krenn V评分在B组最高，在C组最低（p
Ultrasound is commonly used to treat knee osteoarthritis (KOA), which has unique advantages with regard to relieving pain and inflammation as well as delaying cartilage degeneration, but the underlying mechanisms are less clear. The study aimed to investigate the therapeutic effects of ultrasound on vascular endothelial growth factor (VEGF) expression in cartilage, the synovium, and synovial fluid (SF) in a rabbit model of KOA. Twenty-four New Zealand rabbits were randomly divided into ultrasound (group A), sham ultrasound (group B) and noACLT control groups (group C). Six weeks after undergoing anterior cruciate ligament transection (ACLT), group A was treated with ultrasound and group B was treated with sham ultrasound. Two weeks thereafter, the morphology of the synovium and cartilage were observed. Cartilage and synovium were scored using the Mankin scale and Krenn V scores, respectively. VEGF expression in the cartilage, SF, and synovium of ACLT knee joints was analyzed via immunohistochemistry, western blotting, and RT-PCR. Cartilage degeneration and synovitis were the most severe in group B and the least severe in group C. Similarly, Mankin scores and Krenn V scores were highest in group B and lowest in group C (p