A polyaniline-reduced graphene oxide nanocomposite as a redox nanoprobe in a voltammetric DNA biosensor for Mycobacterium tuberculosis
A polyaniline-reduced graphene oxide nanocomposite as a redox nanoprobe in a voltammetric DNA biosensor for Mycobacterium tuberculosis
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聚苯胺还原氧化石墨烯纳米复合材料作为结核分枝杆菌伏安法 DNA 生物传感器中的氧化还原纳米探针
DOI：
10.1007/s00604-017-2184-5
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发表时间：
2017-06-01
影响因子：
5.7
通讯作者：
Bai, Lijuan
中科院分区：
文献类型：
--
作者：
Chen, Yuhan;Li, Youlun;Bai, Lijuan
本文报道了一种用于检测结核分枝杆菌（MTB）特异性DNA序列的生物传感器的构建。一个聚苯胺还原氧化石墨烯（PANI-rGO）复合材料具有良好的电化学活性被用作氧化还原纳米探针的伏安信号的产生。该复合物用金纳米颗粒（AuNPs）装饰，在其上固定信号探针以形成示踪剂标记。目标DNA与示踪标记杂交后，可以明显观察到聚苯胺还原的氧化石墨烯（PANI-rGO）氧化还原探针产生的伏安信号。该生物传感器可在0.1 pM至10 nM浓度范围内检测MTB的特异性IS 6110 DNA序列，检测限低至50 fM（信噪比为3）。生物传感器也是高度特异性的，并且不识别错配。将该方法应用于痰液等临床标本中变性PCR产物的检测，结果与凝胶电泳结果一致。该检测方法为结核分枝杆菌的检测提供了一种通用而强大的工具，如果有合适的分子标记，也可能用于其他病原体。
用于结核分枝杆菌（MTB）超灵敏检测的电化学DNA生物传感器示意图。它使用氧化还原纳米探针（rGO-PANI）进行信号放大。MCH：6-巯基-1-己醇; PANI：聚苯胺; rGO：还原氧化石墨烯; rGO-PANI：聚苯胺-还原氧化石墨烯纳米复合材料。
AbstractThis article describes the construction of a sandwich-type of DNA biosensor for detecting a specific DNA sequence of Mycobacterium tuberculosis (MTB). A polyaniline-reduced graphene oxide (PANI-rGO) composite with favorable electrochemical activity was used as a redox nanoprobe for the generation of the voltammetric signal. The composite was decorated with gold nanoparticles (AuNPs) onto which the signal probe was immobilized to form the tracer label. After hybridization between target DNA and tracer label, the voltammetric signal resulting from the polyaniline-reduced graphene oxide (PANI-rGO) redox probe can be apparently observed. The biosensor can detect the specific IS6110 DNA sequence of MTB in the 0.1 pM to 10 nM concentration range, and the detection limit is as low as 50 fM (at an S/N ratio of 3). The biosensor is also highly specific and does not recognize mismatches. It was applied to the determination of denatured PCR products in clinical samples such as sputum, and the results agreed with those obtained by gel electrophoresis. This assay provides a versatile and powerful tool for detection of MTB, and probably for other pathogens if appropriate molecular markers are available.
Graphical abstractSchematic of an electrochemical DNA biosensor for the ultrasensitive detection of Mycobacterium tuberculosis (MTB). It uses a redox nanoprobe (rGO-PANI) for signal amplification. MCH: 6-mercapto-1-hexanol; PANI: polyaniline; rGO: reduced graphene oxide; rGO-PANI: polyaniline-reduced graphene oxide nanocomposite.