Genome-wide identification and characterization of polygalacturonase genes in Cucumis sativus and Citrullus lanatus.
Genome-wide identification and characterization of polygalacturonase genes in Cucumis sativus and Citrullus lanatus.
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DOI：
10.1016/j.plaphy.2013.11.022
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Youjian Yu;Ying Liang;M. Lv;Jian Wu;G. Lu;Jiashu Cao
中科院分区：
文献类型：
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作者：
Youjian Yu;Ying Liang;M. Lv;Jian Wu;G. Lu;Jiashu Cao
多聚半乳糖醛酸酶（Polygalacturonase，PG，EC3.2.1.15）是与植物细胞壁中果胶网络的修饰相关的水解酶之一，在植物发育所必需的各种细胞分离过程中具有重要作用。植物中PG由一个大的基因家族编码。然而，关于该基因家族在植物发育中的信息仍然有限。在本研究中，53和62推定的PG基因家族成员在黄瓜和西瓜基因组中，分别确定了全基因组搜索，以探讨PG家族的组成，结构和进化的葫芦科作物。结果表明，串联重复可能是导致PG基因在两种作物中扩增的重要因素。系统发育和进化分析表明，PG可分为7个分支，外显子/内含子结构和内含子相位在分支内是保守的，但在分支间是不同的。在拟黄瓜和黄瓜的共同祖先中检测到至少24个祖先PG。实时荧光定量聚合酶链反应（RT-PCR）表达谱分析表明，大多数CsPG在其中一个器官/组织中具有特异性或高表达模式。根据16 CsPG在果实发育中的特异表达模式及其启动子区的膜元件，将其分为果实特异性、胚乳/种子特异性和乙烯反应性3个亚类。通过比较分析，为进一步研究PG基因家族在植物发育过程中的功能提供了基础信息。
Polygalacturonase (PG, EC3.2.1.15), one of the hydrolytic enzymes associated with the modification of pectin network in plant cell wall, has an important role in various cell-separation processes that are essential for plant development. PGs are encoded by a large gene family in plants. However, information on this gene family in plant development remains limited. In the present study, 53 and 62 putative members of the PG gene family in cucumber and watermelon genomes, respectively, were identified by genome-wide search to explore the composition, structure, and evolution of the PG family in Cucurbitaceae crops. The results showed that tandem duplication could be an important factor that contributes to the expansion of the PG genes in the two crops. The phylogenetic and evolutionary analyses suggested thatPGscould be classified into seven clades, and that the exon/intron structures and intron phases were conserved within but divergent between clades. At least 24 ancestral PGs were detected in the common ancestor ofArabidopsisandCucumissativus. Expression profile analysis by quantitative real-time polymerase chain reaction demonstrated that mostCsPGsexhibit specific or high expression pattern in one of the organs/tissues. The 16CsPGsassociated with fruit development could be divided into three subsets based on their specific expression patterns and thecis-elements of fruit-specific, endosperm/seed-specific, and ethylene-responsive exhibited in their promoter regions. Our comparative analysis provided some basic information on the PG gene family, which would be valuable for further functional analysis of the PG genes during plant development.