FORMATION OF THE A-B CIS RING JUNCTION OF ECDYSTEROIDS IN THE LOCUST, SCHISTOCERCA-GREGARIA
FORMATION OF THE A-B CIS RING JUNCTION OF ECDYSTEROIDS IN THE LOCUST, SCHISTOCERCA-GREGARIA
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DOI：
10.1042/bj1940053
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发表时间：
1981-01-01
影响因子：
4.1
通讯作者：
GOODWIN, TW
中科院分区：
文献类型：
--
作者：
DAVIES, TG;DINAN, LN;GOODWIN, TW
本文对蝗虫蜕皮甾体A/B顺环连接的形成机理进行了探讨。gregaria，通过掺入[4- 14 C，3 α- 3H]、[4- 14C，4 α- 3 H]和[4- 14 C，4 β-在5龄幼虫中，[3 H]胆固醇转化为20-羟基蜕皮激素，在成熟的成年雌性卵巢中转化为蜕皮甾体结合物。在这两个系统中，都保留了4 α。蜕皮类固醇中的3 H原子和3 α- 4. beta.- 3 H原子。保留在蜕皮激素中的3 H由[4 α-卵巢系统中的[3 H]胆固醇可能位于C-4。3-氧代-Δ 4中间体可能参与昆虫蜕皮甾类的生物合成。
The mechanism of formation of the A/B cis ring junction of ecdysteroids in the locust, S. gregaria, was investigated by incorporation of [4-14C,3.alpha.-3H], [4-14C,4.alpha.-3H] and [4-14C,4.beta.-3H]cholesterol into 20-hydroxyecdysone in 5th-instar larvae and into ecdysteroid conjugates in ovaries of maturing adult females. In both systems there was retention of the 4.alpha.-3H atom in the ecdysteroid and elimination of the 3.alpha.- and 4.beta.-3H atoms. The 3H retained in the ecdysone formed from [4.alpha.-3H]cholesterol in the ovarian system was probably located at C-4. A 3-oxo-.DELTA.4 intermediate probably is involved in ecdysteroid biosynthesis in insects.