A relaxin‐like gonad‐stimulating peptide identified from the starfish Astropecten scoparius
A relaxin‐like gonad‐stimulating peptide identified from the starfish Astropecten scoparius
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从海星 Astropecten scoparius 中鉴定出一种类似放松的性腺刺激肽
DOI：
10.1002/mrd.23444
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发表时间：
2020
影响因子：
2.5
通讯作者：
Katayama Hidekazu
中科院分区：
文献类型：
--
作者：
Mita Masatoshi;Osugi Tomohiro;Matsubara Shin;Kawada Tsuyoshi;Satake Honoo;Katayama Hidekazu
海星中的松弛素样性腺刺激肽（RGP）是第一个被鉴定出的负责最终配子成熟的无脊椎动物促性腺激素。新近从 Paxillosida 目 Astropecten scoparius 中鉴定出 RGP 直系同源物。 TheA. scopariusRGP (AscRGP) 前体由 354 个碱基对开放阅读框编码，是一种 118 个氨基酸 (aa) 的蛋白质，由信号肽 (26 个氨基酸)、B 链 (21 个氨基酸)、C 肽 (47 个氨基酸) 和 A 链 (24 个氨基酸) 组成。 B 链和 C 肽之间、C 肽和 A 链之间以及 C 肽内存在三个假定的加工位点 (Lys-Arg)。这一结构组织表明，成熟的 AscRGP 由 A 链和 B 链组成，具有两个链间二硫键和一个链内二硫键。 B链的C端残基是Gln-Gly-Arg，它是形成酰胺化C端Gln残基的潜在底物。化学合成了非酰胺化 (AscRGP-GR) 和酰胺化 (AscRGP-NH2) 肽，并使用 A.检查了它们对配子脱落活性的影响。斯科帕里乌斯卵巢。 AscRGP-GR 和 AscRGP-NH2 均以相似的剂量依赖性方式诱导卵母细胞成熟和排卵。这是关于 C 端酰胺化功能性 RGP 的第一份报告。总的来说，这些结果表明 AscRGP-GR 和 AscRGP-NH2 在 A 中充当天然促性腺激素。斯科帕里斯。
A relaxin‐like gonad‐stimulating peptide (RGP) in starfish was the first identified invertebrate gonadotropin responsible for final gamete maturation. An RGP ortholog was newly identified fromAstropecten scopariusof the order Paxillosida. TheA. scopariusRGP (AscRGP) precursor is encoded by a 354 base pair open reading frame and is a 118 amino acid (aa) protein consisting of a signal peptide (26 aa), B‐chain (21 aa), C‐peptide (47 aa), and A‐chain (24 aa). There are three putative processing sites (Lys‐Arg) between the B‐chain and C‐peptide, between the C‐peptide and A‐chain, and within the C‐peptide. This structural organization revealed that the mature AscRGP is composed of A‐ and B‐chains with two interchain disulfide bonds and one intrachain disulfide bond. The C‐terminal residues of the B‐chain are Gln‐Gly‐Arg, which is a potential substrate for formation of an amidated C‐terminal Gln residue. Non‐amidated (AscRGP‐GR) and amidated (AscRGP‐NH2) peptides were chemically synthesized and their effect on gamete shedding activity was examined usingA. scopariusovaries. Both AscRGP‐GR and AscRGP‐NH2induced oocyte maturation and ovulation in similar dose‐dependent manners. This is the first report on a C‐terminally amidated functional RGP. Collectively, these results suggest that AscRGP‐GR and AscRGP‐NH2act as a natural gonadotropic hormone inA. scoparius.