Interaction of G-quadruplex with RecA Protein Studied in Bulk Phase and at the Single-Molecule Level
Interaction of G-quadruplex with RecA Protein Studied in Bulk Phase and at the Single-Molecule Level
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在本体相和单分子水平上研究 G-四链体与 RecA 蛋白的相互作用
DOI：
10.1021/jp4036277
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发表时间：
2013
期刊：
影响因子：
3.3
通讯作者：
S. K. Kim and T. Majima
中科院分区：
文献类型：
--
作者：
A. Tanaka;J. Choi;S. K. Kim and T. Majima
由于在人类基因组中有许多重复DNA序列可用于非B DNA结构，如发夹、三链体、Z-DNA、G-四链体等，因此理解DNA修复蛋白与非B DNA形成序列之间的相互作用非常重要。在此基础上，我们在体相和单分子水平上研究了RecA蛋白与人端粒5′-TAGGG-（TTAGGG）3-TT-3′序列及其形成的G-四链体之间的相互作用。RecA@ssDNA丝是由RecA蛋白与富含G的序列相互作用形成的，K+离子使其解离，解离的富含G的序列迅速折叠成G-quadruplex结构，表明在K+离子存在下，G-quadruplex结构比RecA@ssDNA丝更有利。此外，我们表明，构象的G-四链体，这是异质的RecA的情况下，收敛到特定的G-四链体与一个双链反转环后，协会的RecA蛋白。
As in the human genome there are numerous repeat DNA sequences to adopt into non-B DNA structures such as hairpin, triplex, Z-DNA, G-quadruplex, and so on, an understanding of the interaction between DNA repair proteins and a non-B DNA forming sequence is very important. In this regard, the interaction between RecA protein and human telomeric 5′-TAGGG-(TTAGGG)3-TT-3′ sequence and the G-quadruplex formed from this sequence has been investigated in bulk phase and at the single-molecule level. The RecA@ssDNA filament, which is formed by the interaction between RecA protein and a G-rich sequence, was dissociated by the addition of K+ions, and the dissociated G-rich sequence was quickly folded to a G-quadruplex structure, indicating that the G-quadruplex structure is more favorable than the RecA@ssDNA filament in the presence of K+ions. In addition, we demonstrate that the conformation of the G-quadruplex, which is heterogeneous in the absence of RecA, converged to the specific G-quadruplex with one double-chain-reversal loop upon association of RecA protein.