Three-dimensional distribution of Fos-positive neurons in the supramammillary nucleus of the rat exposed to novel environment
Three-dimensional distribution of Fos-positive neurons in the supramammillary nucleus of the rat exposed to novel environment
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DOI：
10.1016/j.neures.2009.04.013
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发表时间：
2009-08-01
影响因子：
2.9
通讯作者：
Sekino, Yuko
中科院分区：
文献类型：
--
作者：
Ito, Makoto;Shirao, Tomoaki;Sekino, Yuko
下丘脑的乳上核（SLIM）通过不同的纤维连接来调节海马的功能。一些调查表明SuM与焦虑和防御行为有关。然而，SuM的函数是未知的。为了证明在大鼠的生理行为中SuM的空间激活，我们研究了暴露于新环境对SuM的Fos诱导。为了纠正显微镜准备过程中背景不均匀的问题，我们采用了卷积滤波，实现了fos阳性神经元的可靠自动计数，并分析了fos阳性神经元在SuM整个区域的分布。大鼠暴露在一个新的开放区域后，在整个SuM中观察到大量的fos阳性神经元。三维密度图显示，fos阳性神经元的密度在中央SuM中最高，特别是在其核心区域。基于这些结果，我们认为内侧SuM调节防御行为，外侧SuM调节海马的情绪和记忆功能。(C) 2009爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
The supramammillary nucleus (SLIM) in the hypothalamus is proposed to regulate the function of the hippocampus through distinct fiber connection. Several investigations suggest that the SuM is relevant to anxiety and defensive behavior. Function of the SuM, however, is not known exactly. In order to demonstrate the spatial activation of the SuM in physiologically behaving rats, we investigated Fos induction in the SuM by exposure to novel environment. To correct uneven background in microscopic preparations, we applied a convolution filter, resulting in reliable automatic counting of Fos-positive neurons and analyzed the distribution of Fos-positive neurons in the whole region of SuM. A large number of Fos-positive neurons were observed throughout the entire SuM after rats exposed to a novel open field. A three-dimensional density map revealed that density of the Fos-positive neurons was highest in the medial SuM, especially in its core regions. Based on these results we suggest that the medial SuM modulates defensive behavior and that the lateral SuM modulates emotional and memory functions of the hippocampus. (C) 2009 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.