Evaluation of the Antioxidant Abilities of Water-Soluble Biosubstances Using the ORAC-ESR Assay in Combination with the Hydroxyl and Superoxide Radical-Eliminating Abilities
Evaluation of the Antioxidant Abilities of Water-Soluble Biosubstances Using the ORAC-ESR Assay in Combination with the Hydroxyl and Superoxide Radical-Eliminating Abilities
复制标题
使用 ORAC-ESR 测定结合羟基和超氧自由基消除能力评估水溶性生物物质的抗氧化能力
DOI：
10.1007/s00723-013-0491-5
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Nakajima;A.;Masuda;Y.;Matsuda;F.;Tajima;K.;Sameshima;H.;Ikenoue;T.
采用ORAC-电子自旋共振（ESR）法测定了不同水溶性生物物质的氧自由基吸收能力（ORAC）值，并结合羟基和超氧自由基清除能力建立了水溶性生物物质的先进数据库。ORAC-ESR分析是基于2,2 ' -偶氮（2-氨基丙烷）二盐酸盐（AAPH）衍生的烷氧基自由基的自旋捕获与其被底物消除之间的竞争反应。根据烷基氧基自由基的5-（2,2-二甲基-1,3-丙氧基环磷基）-5-甲基-1-吡咯胺-氧化物（CYPMPO）自旋加合物的ESR信号的下降来计算底物对烷基氧基自由基的清除能力。ORAC值的测定是通过测定自旋阱相对于标准自由基消除剂6-羟基-2,5,7,8-四甲基铬-2-羧酸（Trolox）的特异性来完成的。水溶性生物物质的ORAC值[μmol Trolox当量(TE)/g]分为高值组（超过10,000 μmol TE/g）、中值组（400-10,000 μmol TE/g）和低值组（<100 μmol TE/g）。绘制了ORAC值（μmol TE/g）和羟基清除能力(1/[(mg/ml)]和超氧自由基清除能力（1/[(mg/ml)]）的二维图。ORAC值越高的生物物质对超氧化物自由基的清除能力越强（相关因子0.73）。ORAC值与1/[(mg/ml)]值（相关因子0.32）相关性不大。所得的图将为研究食品和药品的抗氧化能力提供一个数据库。
The oxygen radical absorbance capacity (ORAC) values of various water-soluble biosubstances using the ORAC-electron spin resonance (ESR) assay were examined, and an advanced database of water-soluble biosubstances in combination with the hydroxyl and the superoxide radical-eliminating abilities was prepared. The ORAC-ESR assay is based on the competitive reaction between the spin trapping of the 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-derived alkoxyl radical and its elimination by substrate. The alkoxyl radical-eliminating ability of substrate was calculated from the decrease in the ESR signal of the 5-(2,2-dimethyl-1,3-propoxy cyclophosphoryl)-5-methyl-1-pyrrolineN-oxide (CYPMPO) spin adduct of the alkoxyl radical. The determination of ORAC values was performed by determining the specificity of a spin trap relative to a standard radical eliminator, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox). The ORAC values [μmol Trolox equivalent (TE)/g] of water-soluble biosubstances were classified into three groups, the high value group (over 10,000 μmol TE/g), the intermediate value group (400–10,000 μmol TE/g), and the low value group (<100 μmol TE/g). The two-dimensional diagrams for the ORAC values (μmol TE/g) and the hydroxyl and superoxide radical-eliminating abilities (1/[(mg/ml)] and 1/[(mg/ml)], respectively) were depicted. Biosubstances having higher ORAC values had higher superoxide radical-eliminating abilities (the correlation factor 0.73). Little correlation between the ORAC values and the 1/[(mg/ml)] values (the correlation factor 0.32) was observed. The obtained diagrams should provide a database for the study of the antioxidant abilities of foods and pharmaceutics.