A Microfluidic Sensor for Continuous, in Situ Surface Charge Measurement of Single Cells
A Microfluidic Sensor for Continuous, in Situ Surface Charge Measurement of Single Cells
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DOI：
10.1021/acssensors.9b02411
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发表时间：
2020-02-01
期刊：
影响因子：
8.9
通讯作者：
Zhe, Jiang
中科院分区：
文献类型：
--
作者：
Ni, Liwei;Shaik, Rubia;Zhe, Jiang
细胞表面电荷已被认为是一种重要的细胞性质。我们开发了一种基于电阻脉冲传感的微流控传感器，以评估悬浮在连续流中的单细胞的表面电荷和大小。该设备由两个连续的电阻脉冲传感器（RPS）具有相同的尺寸。在两个RPS上施加相反的电场。在RPS中的充电电池被电场加速或减速，因此表现出不同的通过两个RPS的渡越时间。用zeta电位测量电池表面电荷，zeta电位可以用渡越时间差量化。利用RPS产生的脉冲宽度可以准确地检测每个细胞的渡越时间，同时利用脉冲幅度可以计算细胞大小。与传统的电泳光散射方法相比，该装置具有检测单个细胞的表面电荷和尺寸的能力，并且对细胞类型和测试溶液具有高耐受性。三种不同类型的细胞，包括HeLa癌细胞，人皮肤成纤维细胞，和人脐静脉内皮细胞（HUVEC）进行了测试与传感器。结果表明，HeLa细胞与成纤维细胞和HUVECs的zeta电位有显著差异。此外，当用不同浓度的谷氨酰胺处理HeLa细胞时，检测对癌细胞表面电荷的影响。我们的研究结果证明了使用我们的传感器进行细胞类型分选，癌细胞检测和细胞状态分析的巨大潜力。
Cell surface charge has been recognized as an important cellular property. We developed a microfluidic sensor based on resistive pulse sensing to assess surface charge and sizes of single cells suspended in a continuous flow. The device consists of two consecutive resistive pulse sensors (RPSs) with identical dimensions. Opposite electric fields were applied on the two RPSs. A charged cell in the RPSs was accelerated or decelerated by the electric fields and thus exhibited different transit times passing through the two RPSs. The cell surface charge is measured with zeta potential that can be quantified with the transit time difference. The transit time of each cell can be accurately detected with the width of pulses generated by the RPS, while the cell size can be calculated with the pulse magnitude at the same time. This device has the ability to detect surface charges and sizes of individual cells with high tolerance in cell types and testing solutions compared with traditional electrophoretic light scattering methods. Three different types of cells including HeLa cancer cells, human dermal fibroblast cells, and human umbilical vein endothelial cells (HUVECs) were tested with the sensor. Results showed a significant difference of zeta potentials between HeLa cells and fibroblasts or HUVECs. In addition, when HeLa cells were treated with various concentrations of glutamine, the effects on cancer cell surface charge were detected. Our results demonstrated the great potential of using our sensor for cell type sorting, cancer cell detection, and cell status analysis.