Basic study of soft tissue augmentation by adipose-inductive biomaterial
Basic study of soft tissue augmentation by adipose-inductive biomaterial
复制标题
脂肪诱导生物材料软组织填充的基础研究
DOI：
10.1002/jbm.b.33180
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发表时间：
2014
影响因子：
3.4
通讯作者：
Kishi K
中科院分区：
文献类型：
--
作者：
Yazawa M;Mori T;Nakayama Y;Kishi K
肿瘤切除、创伤和先天性畸形的重建手术包括自体组织移植的体积增加。然而，作为供体部位的健康区域受损，自体组织像拼凑物一样转移到受体部位。我们已经尝试在人造生物材料中诱导脂肪生成活性，这种材料可以用注射针注射用于软组织增强。首先，根据增殖因子激活受体γ mRNA的体外表达水平，用脂肪前体细胞检测最佳剂量盐酸吡格列酮。然后调整加入吡格列酮的三文鱼胶原蛋白的剂量，在体内用注射针将三文鱼胶原蛋白注射到小鼠背部。植入后4周，用成熟脂肪细胞代替吡格列酮胶原凝胶，与不含吡格列酮的对照胶原凝胶进行比较。这些结果表明，使用吡格列酮作为脂肪诱导物质的胶原蛋白促进脂肪形成的可能性。©2014 Wiley期刊公司[J]中国生物医学工程学报，2016,31(2):559 - 564。
Reconstructive surgery for tumor resection, trauma, and congenital anomaly involves volume augmentation with autologous tissue transfer. However, a healthy region is damaged as a donor site, and the autologous tissue is transferred like a patchwork to the recipient site. We have attempted to induce adipogenesis activity in artificial biomaterial that is injectable with an injection needle for soft tissue augmentation. First of all, the optimal dose of pioglitazone hydrochloride was examined with adipo‐precursor cells in terms of the proliferator‐activated receptor‐γ mRNA expression levels affected by reagentin vitro. Then, salmon collagen with pioglitazone was adjusted in terms of the dose and the salmon collagen was injected into mouse back using an injection needlein vivo. At 4 weeks after implantation, the pioglitazone collagen gel was substituted by mature adipocytes in comparison with the case for control collagen gel without pioglitazone. These results are indicative of the possibility of promoting adipogenesis using collagen with pioglitazone as an adipose‐inductive substance. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 92–96, 2015.