Identification of a gene involved in the generation of 4-thiouridine in tRNA
Identification of a gene involved in the generation of 4-thiouridine in tRNA
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DOI：
10.1093/nar/26.11.2606
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发表时间：
1998-06-01
影响因子：
14.9
通讯作者：
Paulson, JL
中科院分区：
文献类型：
--
作者：
Mueller, EG;Buck, CJ;Paulson, JL
所有生物在转录后都会修改其核糖核酸的碱基。人们对这些化学变化所起的作用知之甚少，除了极少数例外，关于所涉及的酶的研究更是少之又少。已知功能的一个修饰碱基是某些细菌tRNA第8位的4-硫代尿苷，它用作近紫外光的光传感器。一个参与8位尿苷转化为4-硫代尿苷的基因已经通过遗传筛选而被鉴定，并且它在4-硫代尿苷生成中的作用已被生化证实。同样的基因thil，最近被证明在硫胺素的生物合成中发挥作用。此外，还报道了纯化蛋白的纯化和性质。
All organisms modify the bases of their RNA after transcription. Relatively little is known about the functions that these chemical alterations serve and, with very few exceptions, even less has been established regarding the enzymology involved. One modified base of known function is 4-thiouridine at position 8 of certain bacterial tRNAs, which serves as a photosensor for near-UV light. A gene involved in the conversion of uridine at position 8 into 4-thiouridine has been identified by genetic screening and its role in 4-thiouridine generation has been confirmed biochemically. This same gene, thil, has recently been shown to play a role in thiamin biosynthesis. The purification and characteristics of the purified protein ave also reported.