Expression of HuD protein is essential for initial phase of neuronal differentiation in rat pheochromocytoma PC12 cells
Expression of HuD protein is essential for initial phase of neuronal differentiation in rat pheochromocytoma PC12 cells
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DOI：
10.1006/bbrc.1998.8247
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发表时间：
1998-03-06
影响因子：
3.1
通讯作者：
Kameya, T
中科院分区：
文献类型：
--
作者：
Dobashi, Y;Shoji, M;Kameya, T
HuD是一种神经元特异性rna结合蛋白。本研究检测了神经生长因子介导的PC12细胞分化过程中HuD蛋白表达的变化。随着细胞分化和神经突的延伸，HuD的表达逐渐增加，达到1.5倍，当HuD的表达被反义寡核苷酸抵消时，神经突的延伸被完全抑制，但分化细胞的形态即使在处理后也保持不变。此外，这种形态变化与细胞周期蛋白依赖性激酶2活性的下调密切相关。这些结果表明，HuD在神经元分化的初始阶段起着关键作用。(C) 1998学术出版社。
HuD is a neuronal-specific, RNA-binding protein. Here we examined the change in the expression of HuD protein during nerve growth factor-mediated differentiation of PC12 cells. As cells differentiated and extended neurites, expression of HuD gradually increased up to 1.5-fold, When HuD expression was counteracted by antisense oligonucleotide, neurite extension was completely inhibited, yet the morphology of differentiated cells remained unchanged even after that treatment. Furthermore, this morphological change correlated well with the downregulation of cyclin-dependent kinase 2 activity. These results suggest that the HuD is critically involved in the initial phase of neuronal differentiation. (C) 1998 Academic Press.