Faster Evaluation of Induced Floral Sterilit
Faster Evaluation of Induced Floral Sterilit
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更快地评估诱导花卉灭菌
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
M. Fladung
中科院分区：
文献类型：
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作者：
H. Hoenicka;O. Nowitzki;T. Debener;M. Fladung
摘要转基因树的一个主要问题是转基因通过花粉和种子传播的可能性。在转基因树木品系中引入不育诱导基因是为了减少甚至避免转基因向非转基因杂交物种的基因流。转基因林木的长营养阶段阻碍了对其诱导不育策略的评估。本研究以早花35S：：多叶杨树品系为材料，对其不育结构C-GPDHC：：Vst1进行了快速鉴定。两种转基因方法的结合，一种诱导早花，另一种诱导不育，使这种不育策略在转化两年后得以评估。考虑到森林树种中常见的长达七到二十年的营养期，这是一个非常短的时间。这种方法为评估该植物组的不育机制提供了机会。
Abstract A major concern over the use of transgenic trees is the potential for transgene dispersal through pollen and seeds. The incorporation of sterility inducing genes into transgenic lines of trees has been proposed to reduce or even avoid gene flow of transgenes into non-transgenic interbreeding species. The evaluation of strategies for the induction of sterility in transgenic forest tree species has been hindered by their long vegetative phases. In this study an early flowering 35S::Leafy poplar line was used for the faster evaluation of the sterility construct C-GPDHC::Vst1. The combination of two transgenic approaches, one to induce early flowering and a second for the induction of sterility, allowed evaluation of this sterility strategy two years after transformation. This is a very short period of time considering the long vegetative period of seven to twenty years common in forest tree species. This approach opens opportunities for the assessment of sterility mechanisms for this plant group.