Cyclooxygenase-2 activity is essential for the osseointegration of dental implants
Cyclooxygenase-2 activity is essential for the osseointegration of dental implants
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DOI：
10.1016/j.ijom.2006.11.011
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发表时间：
2007-05-01
影响因子：
2.4
通讯作者：
Takato, T.
中科院分区：
文献类型：
--
作者：
Chikazu, D.;Tomizuka, K.;Takato, T.
本研究的目的是检查环氧合酶 (COX)-2 在将植入物植入小鼠股骨后对骨反应的影响。将直径1.0 mm的钛植入物植入9周龄雄性COX-2野生型(COX-2(+/+))和基因敲除(COX-2(-/-))小鼠的股骨中部。为了进行RNA分析，在植入后0、1、2、4、7和56天处死小鼠。从植入物周围的骨头中提取 RNA。为了进行组织学分析，在治疗后4周和8周处死小鼠，并制备未脱钙切片。进行接触式显微摄影，切片用1％甲苯胺蓝染色进行组织学检查。使用基于计算机的图像分析仪获得组织形态测量，以量化植入物周围新形成的骨以及植入物与骨的接触率。 COX-2(+/+)小鼠中植入物周围的骨中COX-2和骨钙素mRNA的表达被诱导，但COX-2(-/-)小鼠中则没有。在皮质骨中，种植体表面与 COX-2(+/+) 小鼠中新形成的骨板直接接触； COX-2(-/-) 小鼠中新骨形成极少。这些结果表明COX-2在骨整合中发挥重要作用，并为临床上COX-2选择性非甾体抗炎药可能干扰骨整合提供证据。
The aim of this study was to examine the effect of cyclooxygenase (COX)-2 on bone response after the placement of implants in the femurs of mice. titanium implants 1.0 mm in diameter were placed into the middle of the femurs of 9-week-old male COX-2 wild-type (COX-2(+/+)) and knockout (COX-2(-/-)) mice. For RNA analysis, the mice were killed 0, 1, 2, 4, 7 and 56 days after implantation. RNA was extracted from the bone surrounding the implants. For histological analysis, the mice were killed 4 and 8 weeks after treatment, and undecalcified sections were prepared. Contact microradiography was performed, and the sections were stained with 1% toluidine blue for histological examination. Histomorphometric measurements were obtained with a computer-based image analyser to quantify bone newly formed around the implant and the rate of implant-bone contact. Expression of COX-2 and osteocalcin mRNA was induced in bone surrounding implants in COX-2(+/+) mice, but not in COX-2(-/-) mice. In cortical bone, the implant surface was in direct contact with newly formed bone lamellae in COX-2(+/+) mice; new bone formation was minimal in COX-2(-/-) mice. These results suggest that COX-2 plays an essential role in osseointegration and provide evidence that COX-2-selective non-steroidal anti-inflammatory drugs may interfere with osseointegration clinically.