Lipofuscin: Detection and Quantification by Microscopic Techniques
Lipofuscin: Detection and Quantification by Microscopic Techniques
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DOI：
10.1007/978-1-60761-411-1_13
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Grune, Tilman
中科院分区：
文献类型：
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作者：
Jung, Tobias;Hoehn, Annika;Grune, Tilman
由于脂褐素，即所谓的“衰老色素”，在衰老过程中，特别是在肌肉或神经细胞的有丝分裂后衰老中起着重要作用，它成为衰老和应激研究的焦点。在正常衰老过程中，脂褐素以接近线性的方式积累，而在衰老的最后阶段或在一些病理过程的进展中，脂褐素的形成速度可以增加。因此，无论是在衰老过程还是病理过程中，脂褐素都可以作为一种可检测的“标记物”来估计单个细胞的剩余寿命、细胞遭受的长期氧化应激的数量，或在体内或体外量化和鉴定病理过程。为了实现这一点，必须使用一种快速简便的适用的脂褐素检测和定量方法，如荧光显微镜通过“老化色素”测定自身荧光。本文综述了利用脂褐素的物理或化学特性对单细胞中脂褐素进行检测和定量的不同方法。
Since lipofuscin, the so-called "aging pigment", turned out to play a fundamental role in the aging process, particularly in the postmitotic senescence of muscle or neuronal cells, it became a focus of aging and stress research. During normal aging, lipofuscin accumulates in a nearly linear way, whereas its rate of formation can increase in the final stages of senescence or in the progress of several pathologic processes.Thus, both in senescence and pathologic processes, lipofuscin can be used as a detectable "marker" to estimate the remaining lifetime of single cells, the amount of long-term oxidative stress cells were subjected to or to quantify and qualify a pathologic progress in vivo or in vitro. To enable this, a quick and easy applicable method of detection and quantification of lipofuscin has to be used, as is provided by fluorescence microscopy determining the autofluorescence via of the "aging pigment".In this review, we take a look at different methods of detection and quantification of lipofuscin in single cells by using its physical or chemical features.