Cloning and characterization of an arginine vasotocin receptor from the euryhaline flounder Platichthys flesus.
Cloning and characterization of an arginine vasotocin receptor from the euryhaline flounder Platichthys flesus.
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广盐比目鱼 Platichthys flesus 精氨酸催产素受体的克隆和表征。
DOI：
10.1006/gcen.2001.7644
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发表时间：
2001
影响因子：
2.7
通讯作者：
J. Warne
中科院分区：
文献类型：
--
作者：
J. Warne
从硬骨鱼Platichthys flusus的肝脏cDNA文库中筛选出精氨酸催产素（AVT）受体的编码序列。2701-bp的受体序列预测产生一个384-氨基酸的肽，分析表明一个典型的G-蛋白偶联受体的N末端在细胞膜的外表面上的七个跨膜跨越序列。序列分析表明，该序列与Catostomus lastosoni AVT受体（76%）和哺乳动物加压素V（1）型受体（62%）有很高的同源性，而与C.异催产素受体一个双电极电压钳被用来表征受体表达非洲爪蟾卵母细胞。AVT诱导内向电流，该电流在16.7 fmol至5 pmol范围内具有剂量依赖性;异催产素在相同剂量范围内没有影响。哺乳动物血管加压素V（1）型受体激动剂（[Phe（2），Orn（8）] oxytocin）（）诱导内向电流，但不如AVT有效，而哺乳动物血管加压素V（2）型受体激动剂（[Deamino（1），瓦尔（4），D-Arg（8）] AVP）则无作用。用肝素或BAPTA注射卵母细胞抑制对AVT的反应，表明受体与磷脂酶C-磷脂酰肌醇途径有关。北方分析表明，这种AVT受体mRNA的存在，在大脑，肾脏，鳃的比目鱼。
A sequence coding for an arginine vasotocin (AVT) receptor has been identified by the screening of a hepatic cDNA library from the teleost Platichthys flesus. The 2701-bp receptor sequence is predicted to yield a 384-amino acid peptide, analysis of which indicates a seven-transmembrane spanning sequence typical of G-protein-coupled receptors with the N terminus on the outer surface of the cell membrane. Sequence analysis showed this sequence to have a high homology with the Catostomus commersoni AVT receptor (76%) and mammalian vasopressin V(1)-type receptor (62%), but only 55% homology with the C. commersoni isotocin receptor. A two-electrode voltage clamp was used to characterize the receptor expressed in Xenopus laevis oocytes. AVT induced an inward current which was dose dependent over the range 16.7 fmol to 5 pmol; isotocin was without effect over the same dose range. The mammalian vasopressin V(1)-type receptor agonist ([Phe(2), Orn(8)] oxytocin)() induced an inward current but was less potent than AVT, whereas the mammalian vasopressin V(2)-type receptor agonist ([Deamino(1), Val(4), D-Arg(8)] AVP) was without effect. Injection of oocytes with heparin or BAPTA suppressed the response to AVT, indicating receptor linkage to the phospholipase C-phosphatidylinositol pathway. Northern analysis demonstrated the presence of this AVT receptor mRNA in the brain, kidney, and gill of flounder.
影响因子：
3.6
作者：
S. Sladek;Andrea Westerhausen-Larson;James M. Roberts
通讯作者：
S. Sladek;Andrea Westerhausen-Larson;James M. Roberts